- Clone
- MHL-38 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Immunoglobulin light chain lambda
- Isotype
- Mouse IgG2a, κ
- Barcode Sequence
- CAGCCAGTAAGTCAC
Cat # | Size | Price | Quantity Check Availability | ||
---|---|---|---|---|---|
316639 | 10 µg | $369.00 |
The MHL-38 antibody reacts with both soluble and membrane human immunoglobulin light chain lambda (λ). It does not react with human immunoglobulin light chain kappa (κ) or heavy chains. The MHL-38 antibody can be used as primary or secondary reagent for immunofluorescent staining or ELISA analysis.
Product Details
- Verified Reactivity
- Human
- Reported Reactivity
- Baboon, Cynomolgus, Rhesus
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human Ig cocktail
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA
- Preparation
- The antibody was purified by chromatography and conjugated with TotalSeq™-Bn oligomer under optimal conditions.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
- Application
-
SB - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining in formalin-fixed, paraffin-embedded (FFPE) lymphoid tissue, and the oligomer sequence is confirmed by sequencing. TotalSeq™-Bn antibodies are compatible with the 10x Visium CytAssist Gene and Protein Expression Assay.
To maximize performance, it is strongly recommended that the reagent be titrated for each application, and that you centrifuge the antibody dilution at 14,000xg at 2 − 8°C for 10 minutes before use. Carefully pipette out the liquid avoiding the bottom of the tube when handling. To determine and optimize dilutions for the addition of Totalseq™-Bn antibodies into pre-designed antibody panels, refer to 10x Genomics Custom Add-on Antibody Optimization guide.
Buyer is solely responsible for determining whether Buyer has all intellectual property rights that are necessary for Buyer's intended uses of the BioLegend TotalSeq™ products. For example, for any technology platform Buyer uses with TotalSeq™, it is Buyer's sole responsibility to determine whether it has all necessary third party intellectual property rights to use that platform and TotalSeq™ with that platform. - Application Notes
-
Clone MHL-38 is not compatible with Human TruStain FcX (Fc Receptor Blocking Solution) (Cat. No. 422301 & 422302).
- Additional Product Notes
-
TotalSeq™-Bn reagents are designed to profile protein levels following an optimized protocol in spatial transcriptomics. Compatible spatial biology devices (e.g. Imaging System, 10x Genomics Visium Spatial CytAssist Gene and Protein Expression instruments and reagents) and sequencer (e.g. Illumina analyzers) are required. TotalSeq™-B reagents are not compatible with the 10x Genomics Visium system. The complete barcode sequence may be provided upon request. Please contact technical support for more information, or visit TotalSeq™-Bn Reagents for 10x Genomics Visium CytAssist Gene and Protein Assay.
- RRID
-
AB_3097309 (BioLegend Cat. No. 316639)
Antigen Details
- Structure
- Ig family
- Distribution
-
B cells
- Cell Type
- B cells
- Biology Area
- Immunology
- Gene ID
- 3535 View all products for this Gene ID
- UniProt
- View information about Ig light chain lambda on UniProt.org
Other Formats
View All Ig light chain λ Reagents Request Custom ConjugationCompare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human Ig light chain λ
Over night cultured human peripheral blood lymphocytes stain... IHC staining using purified anti-human Ig light chain λ anti... -
Biotin anti-human Ig light chain λ
-
FITC anti-human Ig light chain λ
Human peripheral blood lymphocytes stained with MHL-38 FITC Human peripheral blood lymphocytes stained with MHL-38 FITC -
PE anti-human Ig light chain λ
Human peripheral blood lymphocytes stained with MHL-38 PE -
APC anti-human Ig light chain λ
Human peripheral blood lymphocytes stained with CD19 PE and ... -
Alexa Fluor® 488 anti-human Ig light chain λ
Human peripheral blood lymphocytes stained with MHL-38 Alexa... -
Alexa Fluor® 647 anti-human Ig light chain λ
Human peripheral blood lymphoctes stained with MHL-38 Alexa ... -
Pacific Blue™ anti-human Ig light chain λ
Human peripheral blood lymphocytes stained with CD19 PE and ... -
PerCP/Cyanine5.5 anti-human Ig light chain λ
Human peripheral blood lymphocytes were stained with CD19 PE... -
Purified anti-human Ig light chain λ (Maxpar® Ready)
Human PBMCs stained with 142Nd-anti-CD19 (HIB19) and 151Eu-a... -
PE/Dazzle™ 594 anti-human Ig light chain λ
Human peripheral blood lymphocytes stained with CD19 Brillia... -
PE/Cyanine7 anti-human Ig light chain λ
Human peripheral blood lymphocytes stained with CD19 Brillia... -
APC/Fire™ 750 anti-human Ig light chain λ
Human peripheral blood lymphocytes stained with CD19 Brillia... -
TotalSeq™-A0898 anti-human Ig light chain λ
-
TotalSeq™-C0898 anti-human Ig light chain λ
-
Alexa Fluor® 700 anti-human Ig light chain λ
Human peripheral blood lymphocytes were costained with CD19 ... -
TotalSeq™-B0898 anti-human Ig light chain λ
-
APC/Cyanine7 anti-human Ig light chain λ
Human peripheral blood lymphocytes were stained with CD19 FI... -
TotalSeq™-D0898 anti-human Ig light chain λ
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TotalSeq™-Bn0898 anti-human Ig light chain λ
-
Spark Red™ 718 anti-human Ig light chain λ (Flexi-Fluor™)
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PE anti-human Ig light chain λ
Typical results from overnight cultured human peripheral blo... -
APC anti-human Ig light chain λ
Typical results from overnight cultured human peripheral blo...