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- Clone
- TRA-1-81 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Podocalyxin
- Isotype
- Mouse IgM, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
NCCIT (human teratocarcinoma cell line) stained with TRA-1-81 Alexa Fluor® 647
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 330705 | 25 tests | $165 | ||||
| 330706 | 100 tests | $307 | ||||
The TRA-1-81 antibody reacts with a 200-240 kD cell surface antigen expressed by human embryonic stem cells (ESC), embryonic germ cells (EG), and embryonal carcinoma cells. The expression of TRA-1-81 on human ESC is downregulated upon differentiation. Further studies have shown that TRA-1-81 is a neuroaminidase-resistant carbohydrate epitope expressed on podocalyxin, a member of mucin-like glycoprotein with CD34 family. Podocalyxin is a type I tranmembrane sialoprotein with anti-adhesion property.
Product DetailsProduct Details
- Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human embryonic carcinoma cell line 2102Ep cl.2A6
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
ICC - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
-
Red Laser (633 nm)
- Application Notes
-
Additional reported applications (for the relevant formats) include: immunofluorescence cell staining1, immunoprecipitation1, Western blotting1,2, ELISA1, and immunohistochemistry1 of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded sections.
-
Application References
(PubMed link indicates BioLegend citation) -
- Andrews PW, et al. 1984. Hybridoma. 3:347.
- Schopperle WM and Dewolf WC. 2007. Stem Cells. 25:723.
- Product Citations
- RRID
-
AB_1089245 (BioLegend Cat. No. 330705)
AB_1089242 (BioLegend Cat. No. 330706)
Antigen Details
- Structure
- Mucin-like protein, CD34 family member, 200-240 kD
- Distribution
-
embryonic stem cells (ESC), embryonic germ cells (EG), embryonal carcinoma cells
- Function
- Anti-adhesion
- Cell Type
- Embryonic Stem Cells
- Biology Area
- Cell Biology, Immunology, Stem Cells
- Antigen References
-
1. Andrews PW, et al. 1984. Hybridoma. 3:347.
2. Schopperle WM and Dewolf WC. 2007. Stem Cells. 25:723.
3. Takeda T, et al. 2000. Mol. Biol. Cell. 11:3219. - Gene ID
- 5420 View all products for this Gene ID
- UniProt
- View information about TRA-1-81 on UniProt.org
Related Pages & Pathways
Pathways
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