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FITC anti-human CD66b Antibody

Pricing & Availability
Clone
G10F5 (See other available formats)
Regulatory Status
RUO
Workshop
VI MA81
Other Names
CD67, CGM6, NCA-95, CEACAM8
Isotype
Mouse IgM, κ
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Product Citations
publications
G10F5
Human peripheral whole blood granulocytes stained with G10F5 FITC
  • G10F5
    Human peripheral whole blood granulocytes stained with G10F5 FITC
See FITC spectral data
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305103 25 tests $98
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305104 100 tests $233
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Description

CD66b is a 95-100 kD glycosylphosphatidylinositol (GPI)-linked protein also known as CD67, CGM6, and NCA-95. CD66b is a member of the immunoglobulin superfamily, carcinoembryonic antigen (CEA)-like subfamily. CD66b, expressed on granulocytes, has been reported to induce activation in neutrophils and to be involved in heterophilic adhesion with CD66c.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen, formalin-fixed paraffin-embedded tissue sections, and spatial biology (IBEX)5,6.

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
  3. Norling LV, et al. 2012. Arterioscler Thromb Vasc Biol. 32:1970. PubMed
  4. Meinke P, et al. 2015. Neuroimmunol Discord. 25:127. PubMed
  5. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  6. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Osorio FG et al. 2018. Cell reports. 25(9):2308-2316 . PubMed
  2. Ackermann M, et al. 2018. Nat Commun. 9:5088. PubMed
  3. Rodriguez‐Meira A et al. 2019. Molecular cell. 73(6):1292-1305 . PubMed
  4. Zhao Z, et al. 2019. J Immunol Res. 2019:9561350. PubMed
  5. Kubo M, et al. 2018. Oncol Rep. 39:417. PubMed
  6. Bonegio RG, et al. 2019. J Immunol. 202:675. PubMed
  7. Stojkov D, et al. 2017. J Cell Biol. 216:4073. PubMed
  8. Franco LM, et al. 2019. J Exp Med. 216:384. PubMed
  9. Gomez I, et al. 2020. Nat Commun. 11:214. PubMed
  10. Simon SCS, et al. 2020. Oncoimmunology. 9:1727116. PubMed
  11. Fan Z, et al. 2020. STAR Protoc. 1:100012. PubMed
  12. Nijnik A, et al. 2010. J Immunol. 184:2539. PubMed
  13. Little M, et al. 2012. PLoS One. 7:e28626.. PubMed
  14. Pliyev B, et al. 2011. Cell Immunol. 271:214. PubMed
  15. Heim C, et al. 2015. J Immunol. 194:3861. PubMed
  16. Mueller-Leisse J, et al. 2015. Med Mycol. 53: 622-629. PubMed
  17. SenGupta S, et al. 2016. J Leukoc Biol. 100: 1047 - 1059. PubMed
  18. Holokai L, et al. 2020. Cancers (Basel). 12:00. PubMed
  19. De Domenico E, et al. 2020. STAR Protoc. 1:100233. PubMed
  20. Rodriguez-Meira A, et al. 2020. STAR Protoc. 1:100125. PubMed
  21. Zhang Y, et al. 2020. Oncol Lett. 1.053472222. PubMed
  22. Callahan S, et al. 2020. Cell Microbiol. 22:e13210. PubMed
  23. Pulsifer AR, et al. 2020. Infect Immun. 88:00:00. PubMed
  24. Szabo PA, et al. 2021. Immunity. 54(4):797-814.e6. PubMed
  25. Margaroli C, et al. 2021. Cell Reports Medicine. 2(4):100239. PubMed
  26. Reif T, et al. 2021. Cell Reports Medicine. 2(6):100317. PubMed
  27. Louka E, et al. 2021. Journal of Experimental Medicine. 218(2):. PubMed
  28. Fraccarollo D, et al. 2021. eLife. 0.416666666666667. PubMed
  29. Guo Y, et al. 2021. Exp Ther Med. 353:21. PubMed
  30. Seery V, et al. 2021. EBioMedicine. 67:103357. PubMed
  31. Mao FY, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:395. PubMed
  32. Carre C, et al. 2021. iScience. 24:102970. PubMed
  33. Saha R, et al. 2021. Sci Rep. 11:18849. PubMed
  34. Yang C, et al. 2021. J Immunother Cancer. 9:. PubMed
  35. Ceglia V, et al. 2022. Front Immunol. 12:786144. PubMed
  36. Schimek V, et al. 2022. Cell Death Dis. 13:113. PubMed
  37. Krishnan S, et al. 2021. Clin Exp Immunol. 203:458. PubMed
  38. Sureshchandra S, et al. 2022. Cell Rep. 39:110938. PubMed
  39. Appios A, et al. 2021. Bio Protoc. 11:e4044. PubMed
  40. Miralda I, et al. 2020. Front Immunol. 11:497. PubMed
  41. Ozuna H, et al. 2021. Front Immunol. 12:707096. PubMed
  42. Ceglia V, et al. 2021. J Immunol. 207:2060. PubMed
  43. Lo MW, et al. 2022. Clin Transl Immunology. 11:e1413. PubMed
  44. Hess NJ, et al. 2020. J Immunol. 205:272. PubMed
  45. Kedage V, et al. 2022. MAbs. 14:2040083. PubMed
  46. Kfoury Y, et al. 2021. Cancer Cell. 39:1464. PubMed
  47. Ogishi M, et al. 2023. J Exp Med. 220: . PubMed
  48. Mauracher LM, et al. 2023. Res Pract Thromb Haemost. 7:100126. PubMed
RRID
AB_314495 (BioLegend Cat. No. 305103)
AB_314496 (BioLegend Cat. No. 305104)

Antigen Details

Structure
Ig superfamily, CEA antigen group, GPI-linked glycoprotein, 95-100 kD
Distribution

Granulocytes

Function
Cell adhesion, neutrophil activation
Ligand/Receptor
CD66c
Cell Type
Granulocytes, Neutrophils
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Kuijpers T, et al. 1993. J. Immunol. 151:4934.
2. Kuroki M, et al. 1992. J. Leuk. Biol. 52:551.

Gene ID
1088 View all products for this Gene ID
UniProt
View information about CD66b on UniProt.org

Related FAQs

There are no FAQs for this product.

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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