- Clone
- Poly9023 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- HA epitope tag, HA1, HA2, hemagglutinin, Hemagglutinin HA1 chain, Hemagglutinin HA2 chain, YPYDVPDYA, Hemagglutinin tag
- Previously
-
Covance Catalog# PRB-101P
- Isotype
- Rabbit Polyclonal IgG
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![Purified_HA.11_WB_021315 Purified_HA.11_WB_021315](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/Purified_HA.11_WB_021315.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Western Blot showing specificity of HA.11, clone Poly9023, at 1:1000 and 1:2000. Lane 1: Molecular weight marker. Lane 2: CHO Clone 6. Lane 3: BSA negative control.
The HA tag (hemagglutinin) is an amino acid sequence derived from the human influenza hemagglutinin surface glycoprotein, corresponding to amino acids 98-106. It is commonly used as a tag to facilitate detection, isolation, and purification of proteins. The full amino acid sequence is: YPYDVPDYA.
Product DetailsProduct Details
- Antibody Type
- Polyclonal
- Host Species
- Rabbit
- Immunogen
- Polyclonal antibody HA.11 was raised against the twelve amino acid sequence CYPYDVPDYASL.
- Formulation
- Phosphate-buffered solution + 0.03% thimerosal.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1.0 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
- Application
-
WB, IP
ChIP, ICC, Purification - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting.
The optimal working dilution should be determined for each specific assay condition.
• WB: 1:1,000*
• IP: 1:150 - Application Notes
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This antibody is effective in immunoblotting (WB) and immunoprecipitation (IP) of tagged proteins.
*Our Posi-Tag Control Protein (931301) can be used as a helpful positive control for this antibody.
This antibody recognizes the same hemagglutinin flu epitope YPYDVPDYA as the monoclonal antibody, HA.11 clone 16B12. This polyclonal antibody facilitates epitope tag experiments in murine systems and is useful in experiments involving two proteins labeled with two different tags.
This product may contain other non-IgG subtypes. -
Application References
(PubMed link indicates BioLegend citation) -
- Barrott JJ, et al. 2011. PNAS. 108: 12752. (WB, IP)
- Pecot MY, Malhotra V. 2004. Cell. 116:99.
- Delmas C, et al. 2003. J Biol Chem. 278:12443. (IF)
- Caplan S, et al. 2001. J Cell Biol. 154:109. (IF, WB)
- Yeo C, Whitman M. 2001. Mol Cell 7:949.
- Sander TL, et al. 2000. J Biol Chem.:12857. (WB)
- Bosshart H, et al. 1994. J Cell Biol. 126:1157. (IF, IP)
- Hewawasam G, et al. 2010. Mol Cell. 40:444. (ChIP, WB) PubMed
- Hurov JB, et al. 2004. Curr Biol. 14:736. (IF)
- Shieh AW, et al. 2013. PLoS One. 8:75337. (WB, IF) PubMed
- Hackenbeck T, et al. 2009. Cell Cycle. 8:1386. (ICC)
- Satheshkumar PS, et al. 2013. J Virol. 87:10710.
- Luo R, et al. 2009. Cell Signal. 21:1169. (WB) PubMed
- Berg TJ, et al. 2010. J Biol Chem. 285:35255. (WB) PubMed
- Product Citations
- RRID
-
AB_2734670 (BioLegend Cat. No. 902303)
AB_2565018 (BioLegend Cat. No. 902301)
AB_2565019 (BioLegend Cat. No. 902302)
Antigen Details
- Biology Area
- Cell Biology
- Gene ID
- NA
- UniProt
- View information about HA.11 on UniProt.org
Related Pages & Pathways
Pages
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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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