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Purified anti-mouse CD117 (c-Kit) Antibody

Pricing & Availability
Clone
2B8 (See other available formats)
Regulatory Status
RUO
Other Names
c-KIT, Stem Cell Factor Receptor (SCFR)
Isotype
Rat IgG2b, κ
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Product Citations
publications
2B8_111105
C57BL/6 bone marrow cells stained with 2B8 PE and CD11b FITC
  • 2B8_111105
    C57BL/6 bone marrow cells stained with 2B8 PE and CD11b FITC
Cat # Size Price Quantity Check Availability Save
105801 50 µg $71
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105802 500 µg $233
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Description

CD117 is a 145 kD immunoglobulin superfamily member also known as c-Kit and stem cell factor receptor (SCFR). It is a transmembrane tyrosine-kinase receptor that binds the c-Kit ligand (also known as steel factor, stem cell factor, and mast cell growth factor). CD117 is expressed on hematopoietic stem cells (including multipotent hematopoietic stem cells, progenitors committed to myeloid and/or erythroid lineages, and T and B cell precursors), mast cells, and acute myeloid leukemia (AML) cells. CD117 interaction with its ligand is critical for the development of hematopoietic stem cells.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse bone marrow mast cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of acetone fixed frozen sections2, and spatial biology (IBEX)5,6. The 2B8 antibody does not block c-Kit activity.

Application References

(PubMed link indicates BioLegend citation)
  1. Ikuta K, et al. 1992. P. Natl. Acad. Sci. USA 89:1502. (FC)
  2. Podd BS, et al. 2006. J. Immunol. 176:6532. PubMed (IHC)
  3. Bachelet I, et al. 2008. J. Immunol. 180:6064. PubMed (FC)
  4. Charles N, et al. 2010. Nat. Med. 16:701. PubMed (FC)
  5. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  6. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Martin K,et al. 2017. J Immunol.. 10.4049/jimmunol.1601522. PubMed
  2. Matsuba S, et al. 2017. Front Immunol. . 10.3389/fimmu.2017.01538. PubMed
  3. Crowell PD et al. 2019. Cell Rep. 28(6):1499-1510 . PubMed
  4. Morita S et al. 2017. Cell metabolism. 25(4):883-897 . PubMed
  5. Burton OT, et al. 2018. Clin Exp Allergy. 48:288. PubMed
  6. Sereni L, et al. 2018. J Allergy Clin Immunol. 142:1272. PubMed
  7. Wicki S, et al. 2018. Int J Mol Sci. 1.266666667. PubMed
  8. Verbiest T, et al. 2018. Leukemia. 32:1435. PubMed
  9. Wagner AK, et al. 2017. Nat Commun. 8:15627. PubMed
  10. Chen X, et al. 2019. Methods Mol Biol. 2048:131. PubMed
  11. Batsivari A, et al. 2017. Stem Cell Reports. 8:1549. PubMed
  12. Tsubaki T, et al. 2018. Oncotarget. 9:11209. PubMed
  13. Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
  14. Gu Z, et al. 2019. Cancer Discov. 1.227777778. PubMed
  15. Podd B, et al. 2006. J Immunol. 176:6532. PubMed
  16. Bachelet I, et al. 2008. J Immunol. 180:6064. PubMed
  17. Fournier M, Bijl C 2015. Stem Cells Dev. 24: 2413-2422. PubMed
  18. Coelho P, et al. 2015. Open Bio. 5: 150209. PubMed
  19. Zhong X, et al. 2020. Proc Natl Acad Sci U S A. 8563:117. PubMed
  20. Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
  21. Lin Z, et al. 2021. Stem Cells. 39:240. PubMed
  22. Schuler CF, et al. 2020. Allergy. 75:2279. PubMed
  23. Hsieh HY, et al. 2020. Cancer Sci. 111:2400. PubMed
  24. Russell AL, et al. 2021. Mol Ther Methods Clin Dev. 21:710. PubMed
  25. Liu Y, et al. 2021. Nat Commun. 12:6323. PubMed
  26. Li F, et al. 2020. EMBO J. 39:e103205. PubMed
  27. Van Deren DA, et al. 2022. Development. 149: . PubMed
  28. Campisi L, et al. 2022. Nature. 606:945. PubMed
  29. Tarazi S, et al. 2022. Cell. 185:3290. PubMed
RRID
AB_313210 (BioLegend Cat. No. 105801)
AB_313211 (BioLegend Cat. No. 105802)

Antigen Details

Structure
Ig superfamily, 145 kD
Distribution

Hematopoietic stem cells, AML, mast cells

Function
Growth factor receptor, tyrosine kinase
Ligand/Receptor
Stem Cell Factor (SCF)
Cell Type
Embryonic Stem Cells, Hematopoietic stem and progenitors, Leukemia, Mast cells, Mesenchymal Stem Cells
Biology Area
Immunology, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Galli SJ, et al. 1994. Adv. Immunol. 55:1.
3. Ikuta K, et al. 1992. Annu. Rev. Immunol. 10:759.
4. Besmer P, et al. 1986. Nature 320:415.
5. Witte ON. 1990. Cell 63:5.

Gene ID
16590 View all products for this Gene ID
UniProt
View information about CD117 on UniProt.org
Go To Top Version: 2    Revision Date: 06/28/2013

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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