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Purified anti-mouse CD19 Antibody

Pricing & Availability
Clone
6D5 (See other available formats)
Regulatory Status
RUO
Other Names
B4
Isotype
Rat IgG2a, κ
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Product Citations
publications
6D5_Pure_020608
C57BL/6 mouse splenocytes were stained with purified CD19 (clone 6D5) (filled histogram) or rat IgG2a, κ isotype control (open histogram), followed by anti-rat IgG FITC.
  • 6D5_Pure_020608
    C57BL/6 mouse splenocytes were stained with purified CD19 (clone 6D5) (filled histogram) or rat IgG2a, κ isotype control (open histogram), followed by anti-rat IgG FITC.
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115501 50 µg $42
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115502 500 µg $124
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Description

CD19 is a 95 kD glycoprotein also known as B4. It is a member of the Ig superfamily, expressed on all pro-B to mature B cells (during development) and follicular dendritic cells. Plasma cells do not express CD19. CD19, in association with CD21 and CD81, forms a molecular complex integral to B cell activation.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse CD19-expressing K562 human erythroleukemia cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
CyTOF® - Verified
IHC-F - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence7.

Application References

(PubMed link indicates BioLegend citation)
  1. Shoham T, et al. 2003. J. Immunol. 171:4062. (FC)
  2. Goodyear CS, et al. 2004. J. Immunol. 172:2870. (FC)
  3. Kamimura D, et al. 2006. J. Immunol. 177:306. (FC)
  4. Andoniou CE, et al. 2005. Nat. Immunol. 6:1011. (FC)
  5. Lawson BR, et al. 2007. J. Immunol. 178:5366. (FC)
  6. Phan TG, et al. 2007. Nat. Immunol. 8:992. (FC)
  7. Hayashida K, et al. 2008. J. Biol. Chem. 283:19895. (IF) PubMed
  8. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  9. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  10. Stadnisky MD, et al. 2011. Blood. 117:5133. (FC) PubMed
  11. Perlot T, et al. 2012. J. Immunol. 188:1201. (FC) PubMed
  12. Olive V, et al. 2013. Elife. 2:822. PubMed
  13. Miyai T, et al. 2014. PNAS. 111:11780. PubMed
Product Citations
  1. Ma W, et al. 2017. Sci Rep. . 10.1038/s41598-017-15661-6. PubMed
  2. Oyarce K, et al. 2018. Front Immunol. 9:112. PubMed
  3. Godoy-Calderón MJ, et al. 2018. Oncotarget. 8:11370. PubMed
  4. Gubin MM, et al. 2018. Cell. 175:1014. PubMed
  5. Thulasingam S, et al. 2019. Int J Mol Sci. 20. PubMed
  6. Zhu J et al. 2017. Cell stem cell. 20(3):374-384 . PubMed
  7. Taylor NA, et al. 2017. J Clin Invest. 127:3472. PubMed
  8. Cui X, et al. 2017. J Immunol. 199:4066. PubMed
  9. Parigi SM, et al. 2018. Sci Rep. 0.440277778. PubMed
  10. Hoves S, et al. 2018. J Exp Med. 215:859. PubMed
  11. Manni M, et al. 2018. Nat Immunol. 1.074305556. PubMed
  12. Kikuchi K, et al. 2018. J Immunol. 201:635. PubMed
  13. Yang J, et al. 2019. Front Immunol. 2.492361111. PubMed
  14. Atif SM, et al. 2019. JCI Insight. 4:e125494. PubMed
  15. Lim PJ, et al. 2019. Nat Metab. 0.402083333. PubMed
  16. Chen YQ, et al. 2019. J Immunother Cancer. 0.415277778. PubMed
  17. Leist SR, et al. 2019. PLoS One. 14:e0220126. PubMed
  18. Borges TJ, et al. 2018. Nat Commun. 9:3482. PubMed
  19. Khameneh HJ, et al. 2017. J Immunol. 198:196. PubMed
  20. Field CS, et al. 2018. Oncoimmunology. 7:e1376154. PubMed
  21. Carpenter SM, et al. 2017. PLoS Pathog. 13:e1006704. PubMed
  22. Niss Arfelt K, et al. 2017. Blood. 129:866. PubMed
  23. Roussey JA, et al. 2017. J Immunol. 199:3535. PubMed
  24. Englezou PC, et al. 2018. Mol Ther Nucleic Acids. 12:118. PubMed
  25. Mazor R, et al. 2018. Proc Natl Acad Sci U S A. 115:E733. PubMed
  26. McFarlane AJ, et al. 2017. J Allergy Clin Immunol. 140:1068. PubMed
  27. Nascimento DC, et al. 2017. Nat Commun. 8:14919. PubMed
  28. Majri SS, et al. 2018. J Immunol. 200:110. PubMed
  29. Shin J, et al. 2018. Diabetes. 67:1068. PubMed
  30. Hu J, et al. 2019. Am J Transl Res. 3.043055556. PubMed
  31. Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
  32. Stevens MY, et al. 2020. J Neuroinflammation. 0.899305556. PubMed
  33. Majer O, et al. 2019. Nature. 575:366. PubMed
  34. Hayashida K, et al. 2008. J Biol Chem. 283:19895. PubMed
  35. Stadnisky M, et al. 2011. Blood. 117:5133. PubMed
  36. Penninger T 2012. J Immunol. 188:1201. PubMed
  37. Rozanski C, et al. 2015. J Immunol. 194:4717. PubMed
  38. Goh W, et al. 2020. Cell Rep. 33:108285. PubMed
  39. Korin B, et al. 2020. Sleep. :43. PubMed
  40. Schafflick D, et al. 2020. Nat Commun. 247:11. PubMed
  41. Zelazowska MA, et al. 2020. Life Sci Alliance. :3. PubMed
  42. Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
  43. Joseph R, et al. 2021. Br J Cancer. 125:176. PubMed
  44. Chen HW, et al. 2021. Biomedicines. 9:. PubMed
  45. Sutiwisesak R, et al. 2020. PLoS Pathog. 16:e1009000. PubMed
  46. Boal-Carvalho I, et al. 2020. EMBO Rep. 21:e50421. PubMed
  47. Wan Mohd Zawawi WFA, et al. 2021. Sci Rep. 11:10278. PubMed
  48. Hilligan KL, et al. 2020. Nat Commun. 11:5637. PubMed
  49. Rigo MM, et al. 2020. FEBS J. :. PubMed
  50. Suzuki Y, et al. 2021. FEBS Open Bio. 11:2619. PubMed
  51. Seo SU, et al. 2021. Front Immunol. 12:697162. PubMed
  52. Yan J, et al. 2020. Cancer Immunol Res. 8:356. PubMed
  53. Fernandes V, et al. 2020. PLoS Pathog. 16:e1008464. PubMed
  54. Zhang MH, et al. 2020. Front Immunol. 11:560. PubMed
  55. Hwang JY, et al. 2020. Front Immunol. 11:570661. PubMed
  56. Chen L, et al. 2020. Front Immunol. 11:584458. PubMed
  57. Kästele V, et al. 2021. Mucosal Immunol. 14:717. PubMed
  58. Brownlie D, et al. 2021. J Immunother Cancer. 9:. PubMed
  59. He LD, et al. 2021. EMBO Rep. 22:e52196. PubMed
  60. Tersteegen A, et al. 2021. Infect Immun. 89:. PubMed
  61. You X, et al. 2022. Front Genet. 12:790990. PubMed
  62. Liu CJ, et al. 2020. Neuro Oncol. 22:1276. PubMed
  63. Doll JR, et al. 2020. PLoS Pathog. 16:e1008296. PubMed
  64. Li H, et al. 2022. Cancer Cell. 40:36. PubMed
  65. Valet C, et al. 2022. J Clin Invest. 132: . PubMed
  66. Moore AR, et al. 2022. Cell Rep. 41:111651. PubMed
  67. Potempa M, et al. 2022. J Immunol. 208:1742. PubMed
  68. Hailemichael Y, et al. 2022. Cancer Cell. 40:509. PubMed
  69. Yuan X, et al. 2023. J Periodontol. 94:277. PubMed
RRID
AB_313636 (BioLegend Cat. No. 115501)
AB_313637 (BioLegend Cat. No. 115502)

Antigen Details

Structure
Ig superfamily, associates with CD21 and CD81, 95 kD
Distribution

Pro-B cells to mature B cells (during development), follicular dendritic cells

Function
Modulates B cell activation and differentiation
Ligand/Receptor
CD21, CD81, Leu-13
Cell Type
B cells, Dendritic cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Fearon DT. 1993. Curr. Opin. Immunol. 5:341.
2. Krop I, et al. 1996. Eur. J. Immunol. 26:238.
3. Krop I, et al. 1996. J. Immunol. 157:48.
4. Tedder TF, et al. 1994. Immunol. Today 15:437.

Gene ID
12478 View all products for this Gene ID
UniProt
View information about CD19 on UniProt.org
Go To Top Version: 2    Revision Date: 05/24/2013

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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