- Clone
- L5 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- FLAG tag
- Isotype
- Rat IgG2a, λ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![L5_A647_DYKDDDDK_Tag_Antibody_1_092817 L5_A647_DYKDDDDK_Tag_Antibody_1_092817](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/L5_A647_DYKDDDDK_Tag_Antibody_1_092817.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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HeLa cells transiently transfected with DYKDDDDK tag fused protein were fixed with 4% paraformaldehyde (PFA) for 15 minutes, permeabilized with 0.5% Triton X-100 for 3 minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained with 1:1000 diluted (0.5 µg/ml) Alexa Fluor® 647 anti-DYKDDDDK Tag antibody (red). Nuclei were counterstained with DAPI (blue, Cat. No. 422801). The image was captured with a 40X objective. -
HeLa cells transiently transfected with DYKDDDDK tag fused protein or non-transfected were fixed with 4% paraformaldehyde (PFA) for 15 minutes, permeabilized with 0.5% Triton X-100 for 3 minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained with 1:250 (2 µg/ml), 1:1000 (0.5 µg/ml) and 1:2500 diluted (0.2 µg/ml) Alexa Fluor® 647 anti-DYKDDDDK Tag antibody (red). Nuclei were counterstained with DAPI (blue, Cat. No. 422801). The image was captured with a 40X objective using KEYENCE BZ-X700 fluorescence microscope.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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637315 | 25 µg | 128€ | ||||
637316 | 100 µg | 296€ |
The DYKDDDDK tag, commonly referred to as Sigma®'s FLAG® Tag, is often used as a protein modification in order to simplify the labeling and detection of proteins.This unique amino acid sequence allows for specific antibody detection in western blotting, immunoprecipitation, and immunostaining techniques.Due to the short sequence, this modification is not likely to affect the structure or function of the modified proteins.
Product DetailsProduct Details
- Reactivity
- Epitope tag
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- DYKDDDDK-tagged mouse Langerin
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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ICC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 1:250-1:2500 (0.2 - 2 µg/mL) is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
-
Red Laser (633 nm)
- Application Notes
-
The L5 clone has been demonstrated to have 2-8 fold better sensitivity in WB than another commonly used antibody clone, M2.
- Application References
-
- Park SH, et al. 2008. J Immunol Methods. 331:27.
- Moon SH, et al. 2010. J. Biol Chem. 285:12935. PubMed
- Sasaki M, et al. 2011. J. Biol Chem. 286:39370. PubMed
- Sonder SU, et al. 2012. J Immunol. 188:5906. PubMed
- Jiang Y, et al. 2013. Int Immunol. 25:235. PubMed
- Zuo X, et al. 2014. PLoS One. 9:84748. PubMed
- Toyo-Oak K, et al. 2014. J Neurosci. 34:12168. PubMed
- Product Citations
- RRID
-
AB_2716154 (BioLegend Cat. No. 637315)
AB_2861049 (BioLegend Cat. No. 637316)
Antigen Details
- Biology Area
- Cell Biology
- Antigen References
-
1. Einhauer A. 2001. J. Biochem. Biophys. Methods. 49:455.
2. Knappik A and Pluckthun A. 1994. Biotechniques. 17:754. - Gene ID
- NA
- UniProt
- View information about DYKDDDDK on UniProt.org
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