- Clone
- L5 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- FLAG tag
- Isotype
- Rat IgG2a, λ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![L5_A488_DYKDDDDK_ICC_Antibody_032918 L5_A488_DYKDDDDK_ICC_Antibody_032918](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/L5_A488_DYKDDDDK_ICC_Antibody_032918.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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HeLa cells non-transfected (A) or transfected with DYKDDDDK Tag fused protein (B and C) were fixed with 4% paraformaldehyde (PFA) for 10 minutes, permeabilized with 0.5% Triton X-100 for 3 minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained overnight at 4 degrees with 1: 250 diluted (2 µg/ml, Figure A and B) and 1: 1000 diluted (0.5 µg/ml) Alexa Fluor® 488 anti- DYKDDDDK Tag (Figure C, green). Nuclei were counterstained with DAPI (blue). The image was captured with a 40X objective.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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637317 | 25 µg | 128€ | ||||
637318 | 100 µg | 296€ |
The DYKDDDDK tag, commonly referred to as Sigma®'s FLAG® Tag, is often used as a protein modification in order to simplify the labeling and detection of proteins.This unique amino acid sequence allows for specific antibody detection in western blotting, immunoprecipitation, and immunostaining techniques.Due to the short sequence, this modification is not likely to affect the structure or function of the modified proteins.
Product DetailsProduct Details
- Reactivity
- Epitope tag
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- DYKDDDDK-tagged mouse Langerin
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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ICC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 0.5 - 2.0 μg/ml (1:250 - 1:1000 dilution) is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
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Blue Laser (488 nm)
- Application Notes
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The L5 clone has been demonstrated to have 2-8 fold better sensitivity in WB than another commonly used antibody clone, M2.
- Application References
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- Park SH, et al. 2008. J Immunol Methods. 331:27.
- Moon SH, et al. 2010. J. Biol Chem. 285:12935. PubMed
- Sasaki M, et al. 2011. J. Biol Chem. 286:39370. PubMed
- Sonder SU, et al. 2012. J Immunol. 188:5906. PubMed
- Jiang Y, et al. 2013. Int Immunol. 25:235. PubMed
- Zuo X, et al. 2014. PLoS One. 9:84748. PubMed
- Toyo-Oak K, et al. 2014. J Neurosci. 34:12168. PubMed
- Product Citations
- RRID
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AB_2728470 (BioLegend Cat. No. 637317)
AB_2810690 (BioLegend Cat. No. 637318)
Antigen Details
- Biology Area
- Cell Biology
- Antigen References
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1. Einhauer A. 2001. J. Biochem. Biophys. Methods. 49:455.
2. Knappik A and Pluckthun A. 1994. Biotechniques. 17:754. - Gene ID
- NA
- UniProt
- View information about DYKDDDDK on UniProt.org
Related Pages & Pathways
Pages
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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