- Clone
- 87G (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Human Leukocyte Antigen-G
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![87G_APC_032311 87G_APC_032311](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/87G_APC_032311.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human cytotrophoblastic cell line (JEG-3) was stained with HLA-G (clone 87G) APC (filled histogram) or mouse IgG2a APC isotype control (open histogram).
Cat # | Size | Price | Save |
---|---|---|---|
335909 | 25 tests | ¥31,240 | |
335910 | 100 tests | ¥74,360 |
HLA-G is a nonclassical MHC class I (MHC-Ib) molecule structurally related to MHC class Ia (HLA-A,B, C). There are seven isoforms of this molecule, including membrane bound HLA-G1, -G2, -G3 and -G4 and soluble HLA-G5, -G6, and -G7. HLA-G is primarily expressed on trophoblast cells. Its expression is also found on thymic epithelial cells, cytokine-activated moncytes, macrophages/dendritic cells during viral infection, and in various tumors. HLA-G exerts its inhibitory functions to regulate immune tolerance via interaction with inhibitory receptors ILT2(CD85j), ILT4(CD85d), and KIR2DL4(CD158d), which is expressed on NK cells, T cells, monocytes, dendritic cells, and B cells. HLA-G is also able to bind CD8α/α, which may mediate positive and/or negative selection in thymus.
Product DetailsProduct Details
- Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- HLA-G transfected cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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FC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
- Excitation Laser
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Red Laser (633 nm)
- Application Notes
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87G mAb reacts with isoforms of HLA-G1 and -G5. Additional reported applications (for the relevant formats) include: restoring HLA-G mediated suppression of allo-T cell proliferation and immunohistochemical staining of frozen tissue sections. Clone 87G is reported not to bind HLA-G in aldehyde fixed samples.4
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Application References
(PubMed link indicates BioLegend citation) -
- Odum N, et al. 1991. Eur. J. Immunol. 21:2121.
- Lila N, et al. 2001. P. Natl. Acad. Sci. USA 98:12150.
- Lila N, et al. 2002. Circulation. 105:1949.
- Blaschitz A, et al. 2000. Hum. Immunol. 61:1074.
- Balsamo M, et al. 2012. Eur J. Immunol. 42:1833. PubMed
- Product Citations
- RRID
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AB_10900805 (BioLegend Cat. No. 335909)
AB_10898111 (BioLegend Cat. No. 335910)
Antigen Details
- Structure
- Ig
- Distribution
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Trophoblast cells, thymic epithelial cells, cytokine-activated moncytes, macrophages/dendritic cells during viral infection, various tumors
- Function
- Inhibit NK and T cell-mediated cytolysis and T cell proliferation, induce apoptosis of activated CD8+ T cells, induce Th2 cytokine production
- Ligand/Receptor
- ILT2(CD85j), ILT4(CD85d), KIR2DL4(CD158d), CD8α/α
- Cell Type
- Dendritic cells, Epithelial cells, Macrophages, Monocytes
- Biology Area
- Immunology
- Molecular Family
- MHC Antigens
- Antigen References
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1. Hunt JS, et al. 2005. FASEB J. 19:681.
2. Carosella ED, et al. 2008. Blood 111:4862. - Gene ID
- 3135 View all products for this Gene ID
- UniProt
- View information about HLA-G on UniProt.org
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