- Clone
- 63D3 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Monocyte differentiation antigen CD14, myeloid cell-specific leucine-rich glycoprotein, LPS receptor
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![63D3_BV605_CD14_Antibody_1009117 63D3_BV605_CD14_Antibody_1009117](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/63D3_BV605_CD14_Antibody_1009117.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human peripheral blood monocytes were stained with Brilliant Violet™ 605 anti-human CD14 (clone 63D3, closed histogram) or Brilliant Violet™ 605 mouse IgG1, κ isotype control (open histogram).
Cat # | Size | Price | Save |
---|---|---|---|
367125 | 25 tests | ¥45,980 | |
367126 | 100 tests | ¥90,860 |
CD14 is a 53-55 kD glycosylphosphatidylinositol (GPI)-linked membrane glycoprotein that is also known as the LPS receptor. CD14 is expressed at high levels on monocytes and macrophages, and at lower levels on granulocytes. Some dendritic cell populations such as interfollicular dendritic cells, reticular dendritic cells, and Langerhans cells have also been reported to express CD14. As a high-affinity receptor for LPS, CD14 is involved in the clearance of gram-negative pathogens and in the upregulation of adhesion molecules and cytokine expression in monocytes and neutrophils.
Product DetailsProduct Details
- Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Purified human peripheral blood monocytes.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 605™ under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Brilliant Violet 605™ excites at 405 nm and emits at 603 nm. The bandpass filter 610/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 605™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
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Violet Laser (405 nm)
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Application References
(PubMed link indicates BioLegend citation) -
- Fridlender ZG, et al. 1999. Hum. Immunol. 11:1028.
- Devitt A, et al. 1998. Nature 6675:505.
- Product Citations
- RRID
-
AB_2716230 (BioLegend Cat. No. 367125)
AB_2716231 (BioLegend Cat. No. 367126)
Antigen Details
- Structure
- GPI-linked membrane glycoprotein.
- Distribution
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Monocytes, macrophages, dendritic cells, and granulocytes.
- Function
- LPS receptor, clearance of Gram-negative pathogens.
- Interaction
- LPS.
- Ligand/Receptor
- LPS.
- Cell Type
- Dendritic cells, Granulocytes, Macrophages, Monocytes, Neutrophils
- Biology Area
- Cell Biology, Immunology, Neuroinflammation, Neuroscience
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
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- Stocks SC, et al. 1990. Biochem. J. 268:275.
- Wright SD, et al. 1990. Science 4975:1431.
- Gene ID
- 929 View all products for this Gene ID
- UniProt
- View information about CD14 on UniProt.org
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