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PE/Cyanine5 anti-human CD95 (Fas) Antibody

Pricing & Availability
Clone
DX2 (See other available formats)
Regulatory Status
RUO
Workshop
VI C-64
Other Names
Fas, APO-1, TNFRSF6
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
DX2_PECy5_070507
Human peripheral blood lymphocytes stained with DX2 PE/Cyanine5
  • DX2_PECy5_070507
    Human peripheral blood lymphocytes stained with DX2 PE/Cyanine5
See PE/Cyanine5 spectral data
Cat # Size Price Save
305610 100 tests ¥62,260
Description

CD95 is a 45 kD single chain type I glycoprotein also known as Fas, APO-1, and TNFRSF6. It is a member of the TNF receptor superfamily. CD95 is expressed on T and B lymphocytes, monocytes, neutrophils, and fibroblasts. CD95 expression is upregulated by activation. The extracellular region of CD95 binds to CD178 (Fas ligand). CD178 binding to CD95 induces apoptosis and has been shown to play a role in the maintenance of peripheral tolerance.

Product Details
Technical data sheet

Product Details

Reactivity
Human,Cynomolgus,Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
CD95 transfected L cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine5 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The DX2 antibody is useful for inducing apoptosis of Fas-positive cells. Additional reported applications (for the relevant formats) include: in vitro induction of apoptosis3 (DX2 antibody is required to be cross-linked for effective induction of apoptosis) and immunohistochemical staining4,5 of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded tissue sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 305655 and 305656).

Note: EOS9.1 antibody can induce apoptosis without cross-linking.

Additional Product Notes
BioLegend is in the process of converting the name PE/Cy5 to PE/Cyanine5. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine5 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. Eds.1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. New York.
  3. Cifone M, et al. 1994. J. Exp. Med. 180:1547. (Apop)
  4. Zietz C, et al. 2001. Am. J. Pathol. 159:963. (IHC)
  5. Sergi C, et al. 2000. Am. J. Pathol. 156:1589. (IHC)
  6. Xie S, et al. 2010. J. Immunol. 184:2289. (FC) PubMed
  7. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  8. Sestak K, et al. 2007. Vet. Immunol. Immunopathol. 119:21.
  9. Rout N, et al. 2010. PLoS One 5:e9787. (FC)
  10. Dixit N, et al. 2012. J. Immunol. 189:5954. PubMed
Product Citations
  1. Watanabe K, et al. 2018. JCI Insight. 3. PubMed
  2. Han SJ et al. 2017. Immunity. 47(6):1154-1168 . PubMed
  3. Cortés–Rubio CN, et al. 2019. Clin Epigenetics. 11:134. PubMed
  4. Lakhashe S, et al. 2011. PLoS One. 6:e22010. PubMed
  5. Wang Y, et al. 2014. Cytotherapy. 16:122. PubMed
  6. Tabler C, et al. 2014. J Virol. 88:4976. PubMed
  7. Bolton D, et al. 2016. J Virol. 90: 1321 - 1332. PubMed
  8. Chowdhury A, et al. 2015. J Virol. 89: 8677-8686. PubMed
  9. Szabo PA, et al. 2021. Immunity. 54(4):797-814.e6. PubMed
  10. Lozano-Rodríguez R, et al. 2022. Cell Rep. 38:110235. PubMed
RRID
AB_314548 (BioLegend Cat. No. 305610)

Antigen Details

Structure
TNFR superfamily, type I transmembrane protein, 45 kD
Distribution

T cells and B cells, monocytes, neutrophils, fibroblasts, expression level upregulated on activated lymphocytes

Function
Mediates apoptosis
Ligand/Receptor
Fas ligand (CD178)
Cell Type
B cells, Fibroblasts, Lymphocytes, Monocytes, Neutrophils, T cells
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Neuroscience
Molecular Family
CD Molecules
Antigen References

1. Krammer P, et al. 1994. Immunol. Rev. 142:175.
2. Nagata S, et al. 1995. Science 267:1449.

Gene ID
355 View all products for this Gene ID
UniProt
View information about CD95 on UniProt.org
Go To Top Version: 2    Revision Date: 08/08/2016

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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