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PE/Cyanine7 anti-human CD45RO Antibody

Pricing & Availability
Clone
UCHL1 (See other available formats)
Regulatory Status
RUO
Workshop
IV N31
Other Names
CD45RO
Isotype
Mouse IgG2a, κ
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Product Citations
publications
UCHL1_PECy7_1_022712
Human peripheral blood lymphocytes were stained with CD45RA Brilliant Violet 421™ and CD45RO (clone UCHL1) PE/Cyanine7 (top) or mouse IgG2a, κ PE/Cyanine7 isotype control (bottom). Data shown was gated on the CD3+ population.
  • UCHL1_PECy7_1_022712
    Human peripheral blood lymphocytes were stained with CD45RA Brilliant Violet 421™ and CD45RO (clone UCHL1) PE/Cyanine7 (top) or mouse IgG2a, κ PE/Cyanine7 isotype control (bottom). Data shown was gated on the CD3+ population.
  • UCHL1_PECy7_2_022712
See PE/Cyanine7 spectral data
Cat # Size Price Save
304229 25 tests ¥26,170
304230 100 tests ¥61,770
Description

CD45RO is a 180 kD single chain membrane glycoprotein. It is a splice variant of tyrosine phosphatase CD45, lacking the A, B, and C determinants. The CD45RO isoform is expressed on activated and memory T cells, some B cell subsets, activated monocytes/macrophages, and granulocytes. CD45RO enhances both T cell receptor and B cell receptor signaling mediated activation. CD45 and its isoforms non-covalently associate with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4. CD45 has also been reported to bind galectin-1 and CD22. CD45 isoform expression can change in response to cytokines.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
IL-2 dependent T cell line, CA1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The UCHL1 antibody is commonly used in combination with antibodies against CD45RA to discern memory and naïve T cells. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections5 and formalin-fixed paraffin-embedded tissue sections4, Western blotting2, and immunoprecipitation3.

Additional Product Notes
BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York. (FC)
  2. Ishii T, et al. 2001. P. Natl. Acad. Sci. USA 98:12138. (WB)
  3. Ponsford M, et al. 2001. Clin. Exp. Immunol. 124:315. (IP)
  4. Yamada M, et al. 1996. Stroke 27:1155. (IHC)
  5. Sakkas LI, et al. 1998. Clin. Diagn. Lab. Immunol. 5:430. (IHC)
  6. Baba N, et al. 2010. Int. Immunol. 22:237. PubMed
  7. Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
  8. Weiss L, et al. 2010. P. Natl. Acad. Sci. USA 107:10632. PubMed
  9. Wu YY, et al. 2007. Infect. Immun. 75:4357. PubMed
  10. Mozaffarian N, et al. 2008. Rheumatology 47:1335. PubMed
  11. Roque S, et al. 2007. J. Immunol. 178:8028. PubMed
  12. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  13. Smith SH, et al. 1986. Immunology 58:63. (Immunogen)
  14. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
Product Citations
  1. Audigé A, et al. 2017. BMC Immunology. 10.1186/s12865-017-0209-9. PubMed
  2. Huang L, et al. 2017. Immun Ageing. 10.1186/s12979-017-0096-1. PubMed
  3. Cao W, et al. 2017. Immunity. 47:1182. PubMed
  4. Gordeychuk IV, et al. 2019. Acta Naturae. 10:63. PubMed
  5. Kraig E, et al. 2018. Exp Gerontol. 105:53. PubMed
  6. Mann EH, et al. 2019. J Immunol. 202:684. PubMed
  7. Zhai Y, et al. 2018. Autophagy. 1.714583333. PubMed
  8. Jiang W, et al. 2014. J Virol. 88:5369. PubMed
  9. Leeansyah E, et al. 2014. Nat Commun. 5:3143. PubMed
  10. Traitanon O, et al. 2015. PLoS One. 10:129658. PubMed
  11. Delmas A, et al. 2016. PLoS One. 11: 0163305. PubMed
  12. Kieffer T, et al. 2017. J Reprod Immunol. 10.1016/j.jri.2016.11.004. PubMed
  13. Koh WH, et al. 2020. STAR Protoc. 1:100203. PubMed
  14. Gurusamy D, et al. 2020. Cancer Cell. 37(6):818-833.e9. PubMed
  15. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  16. Zhou WJ, et al. 2021. Theranostics. 11:5330. PubMed
  17. Müller TR, et al. 2021. Cell Rep Med. 2:100374. PubMed
  18. Beatson RE, et al. 2021. Cell Rep Med. 2:100473. PubMed
  19. Zeng Q, et al. 2022. Front Immunol. 13:740805. PubMed
  20. Hackstein CP, et al. 2022. Nat Commun. 13:7472. PubMed
  21. Wu L, et al. 2023. Cell Rep Med. 4:100917. PubMed
  22. Cheng H, et al. 2023. Nat Metab. 5:314. PubMed
RRID
AB_11203903 (BioLegend Cat. No. 304229)
AB_11203900 (BioLegend Cat. No. 304230)

Antigen Details

Structure
Tyrosine phosphatases, type I transmembrane, 180 kD (isoform of CD45 containing none of the A, B, or C determinants)
Distribution

Activated and memory T cells, B cell subsets, monocytes, macrophages, granulocytes

Function
Enhances TCR and BCR signaling
Ligand/Receptor
CD22
Cell Type
B cells, Granulocytes, Macrophages, Mesenchymal Stem Cells, Monocytes, T cells, Tregs
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Thomas M. 1989. Annu. Rev. Immunol. 7:339.
2. Trowbridge I, et al. 1994. Annu. Rev. Immunol. 12:85.

Gene ID
5788 View all products for this Gene ID
UniProt
View information about CD45RO on UniProt.org
Go To Top Version: 2    Revision Date: 07/23/2013

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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