Loading...

PE/Cyanine7 anti-mouse CD335 (NKp46) Antibody

Pricing & Availability
Clone
29A1.4 (See other available formats)
Regulatory Status
RUO
Other Names
NKp46, NCR1
Isotype
Rat IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
29A1.4_PECy7_1_060412
C57/BL6 mouse splenocytes were stained with NK1.1 Brilliant Violet 421™ and CD335 (clone 29A1.4) PE/Cyanine7 (top) or rat IgG2a, κ isotype control PE/Cyanine7 (bottom).
  • 29A1.4_PECy7_1_060412
    C57/BL6 mouse splenocytes were stained with NK1.1 Brilliant Violet 421™ and CD335 (clone 29A1.4) PE/Cyanine7 (top) or rat IgG2a, κ isotype control PE/Cyanine7 (bottom).
  • 29A1.4_PECy7_2_060412
See PE/Cyanine7 spectral data
Cat # Size Price Save
137617 25 µg ¥25,100
137618 100 µg ¥64,800
Description

CD335, also known as NKp46, is a single-pass type I membrane protein of 46 kD. It belongs to the natural cytotoxicity receptor (NCR) family and contains two Ig-like (immunoglobulin-like) domains. It's expression is restricted to NK cells and a subset of NKT cells; it's not expressed in CD1d-restricted NKT cells. CD335 is a receptor for viral hemagglutinins and heparan sulfate proteoglycans and is involved in NK cell activation.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
NKP46-IgG1 Fc fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume.  It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue sections1,2 and in vitro activation of NK cells1.

Additional Product Notes
BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Walzer T, et al. 2007. P. Natl. Acad. Sci. USA 104:3384. (FC, Activ)
  2. Walzer T, et al. 2007. Nat. Immunol. 8:1337. (FC, Activ)
  3. Guerriero JL, et al. 2011. J. Immunol. 186:3517. (IHC) PubMed
Product Citations
  1. Feng J,et al. 2017. Nat Commun. . 10.1038/s41467-017-01056-8. PubMed
  2. Isvoranu G, et al. 2019. Oncol Lett. 17:4197. PubMed
  3. Koyama M et al. 2019. Immunity. 51(5):885-898 . PubMed
  4. Westrich JA, et al. 2019. Oncogene. 38:7166. PubMed
  5. Koyama M, et al. 2015. J Exp Med. 212: 1303 - 1321. PubMed
  6. Victorino F, et al. 2015. J Immunol. 195: 4973 - 4985. PubMed
  7. Frasch S, et al. 2016. J Immunol. 197: 1425 - 1434. PubMed
  8. Fachi JL, et al. 2020. J Exp Med. 217:00:00. PubMed
  9. Ni J, et al. 2020. Immunity. 52(6):1075-1087.e8. PubMed
  10. McFarland AP, et al. 2021. Immunity. 54(6):1320-1337.e4. PubMed
  11. Molina-Sanchez P, et al. 2020. Gastroenterology. 2203:159. PubMed
  12. Reinfeld BI, et al. 2021. Nature. 593:282. PubMed
  13. Xu B, et al. 2021. Nat Commun. 12:5908. PubMed
  14. Mincham KT, et al. 2021. Front Immunol. 11:601494. PubMed
  15. Stegelmeier AA, et al. 2022. Biomedicines. 10:. PubMed
  16. Markov SD, et al. 2021. Mol Cancer Ther. 20:2457. PubMed
  17. Tian L, et al. 2022. Clin Cancer Res. 28:201. PubMed
  18. Darragh LB, et al. 2022. Nat Commun. 13:7015. PubMed
  19. Wolf KG, et al. 2022. JVS Vasc Sci. 3:336. PubMed
  20. Millar FR, et al. 2022. Cell Rep. 41:111596. PubMed
  21. Liang Y, et al. 2022. Theranostics. 12:7729. PubMed
  22. Chan L, et al. 2023. Int J Mol Sci. 24: . PubMed
  23. Fueyo-González F, et al. 2022. Immunity. 55:459. PubMed
  24. Tian L, et al. 2022. Nat Cancer. 3:1318. PubMed
RRID
AB_11218594 (BioLegend Cat. No. 137617)
AB_11219186 (BioLegend Cat. No. 137618)

Antigen Details

Structure
Single-pass type I membrane protein, 46 kD; belongs to the natural cytotoxicity receptor (NCR) family; contains 2 Ig-like (immunoglobulin-like) domains
Distribution

Mature and immature NK cells, subset of NKT cells, but not on CD1d-restricted NKT cells

Function
NK cells activation
Ligand/Receptor
Viral hemagglutinins, heparan sulfate proteoglycans
Cell Type
NK cells, NKT cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules
Antigen References

1. Colucci F and Cilio CM. 2010. Nat. Immunol. 125:60.
2. Caligiuri MA. 2008. Blood 112:461.
3. Colonna M. 2009. Immunity 31:15.

Gene ID
17086 View all products for this Gene ID
UniProt
View information about CD335 on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account