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PerCP anti-human CD45 Antibody

Pricing & Availability
Clone
HI30 (See other available formats)
Regulatory Status
RUO
Workshop
IV N816
Other Names
LCA, T200
Isotype
Mouse IgG1, κ
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Product Citations
publications
HI30_PerCP_020708
Human peripheral blood lymphocytes stained with HI30 PerCP
  • HI30_PerCP_020708
    Human peripheral blood lymphocytes stained with HI30 PerCP
See PerCP spectral data
Cat # Size Price Save
304025 25 tests ¥33,660
304026 100 tests ¥73,700
Description

CD45 is a 180-240 kD single chain type I membrane glycoprotein also known as leukocyte common antigen (LCA) and T200. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes and platelets. CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation. CD45 plays a critical role in T and B cell antigen receptor-mediated activation by dephosphorylating substrates including p56Lck, p59Fyn, and other Src family kinases. CD45 non-covalently associates with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to bind galectin-1 and to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PerCP has a maximum absorption of 482 nm and a maximum emission of 675 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded tissue sections9, inhibition of CD45 functions4, immunofluorescence11, Western blotting3, and spatial biology (IBEX)16,17.

It was found that the HI30 clone and the 2D1 clone can cross block each other's binding.

Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  2. Kishihara K, et al. 1993. Cell 74:143.
  3. Esser M, et al. 2001. J. Virol. 75:6173. (WB)
  4. Yamada T, et al. 2002. J. Biol. Chem. 277:28830.
  5. Nagano M, et al. 2007. Blood 110:151.
  6. Jiang Q, et al. 2008. Blood 112:2858. PubMed
  7. Morozov A, et al. 2010. Clin Cancer Res. 16:5630. PubMed
  8. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  9. Friedman T, et al. 1999. J. Immunol. 162:5256. (IHC)
  10. Oeztuerk-Winder F, et al. 2012. EMBO J. 31:3431. (FC) PubMed
  11. Rees LE, et al. 2003. Clin. Exp. Immunol. 134:497. (IF)
  12. Lee J, et al. 2015. J Exp Med. 212:385. PubMed
  13. Breton G, et al. 2015. J Exp Med. 212:401. PubMed
  14. Marquardt N, et al. 2015. J Immunol. 6:2467. PubMed
  15. Bushway ME, et al. 2014. Biol Reprod. 90(5): 110. (IF) PubMed
  16. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  17. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Audigé A, et al. 2017. BMC Immunology. 10.1186/s12865-017-0209-9. PubMed
  2. Schelker RC, et al. 2018. Hematology. 1.192361111. PubMed
  3. Fournier N, et al. 2018. MAbs. 10:651. PubMed
  4. Salvany‐Celades M et al. 2019. Cell Rep. 27(9):2537-2547 . PubMed
  5. Zhong W et al. 2019. Cell reports. 26(8):2166-2177 . PubMed
  6. Dos Santos JC, et al. 2019. Cell Rep. 28:2659. PubMed
  7. Ueda M, et al. 2019. Biol Blood Marrow Transplant. 25:1122. PubMed
  8. Cirovic B, et al. 2020. Cell Host & Microbe. 28(2):322-334. PubMed
  9. Zhu Y, et al. 2019. Cell Stem Cell. 25:542. PubMed
  10. Saraiva DP, et al. 2020. Front Oncol. 1.488194444. PubMed
  11. Saraiva DP, et al. 2018. Front Immunol. 2.184027778. PubMed
  12. Laing AG, et al. 2020. Nat Med. 26:1623. PubMed
  13. Leach SM, et al. 2020. Cell Rep. 33:108337. PubMed
  14. Le J, et al. 2020. Immunity. 52(6):1105-1118.e9. PubMed
  15. Abdul-Jawad S, et al. 2021. Cancer Cell. 39(2):257-275.e6. PubMed
  16. Szabo PA, et al. 2021. Immunity. 54(4):797-814.e6. PubMed
  17. Parent AV, et al. 2021. Cell Reports. 36(7):109538. PubMed
  18. Witkowski MT, et al. 2020. Cancer Cell. 37:867. PubMed
  19. Jundi B, et al. 2021. JCI Insight. 6:e148866. PubMed
  20. Zhan Y, et al. 2021. JCI Insight. 6:. PubMed
  21. Li YR, et al. 2021. Cell Rep Med. 2:100449. PubMed
  22. Zhang P, et al. 2022. Signal Transduct Target Ther. 7:46. PubMed
  23. Felker S, et al. 2022. JCI Insight. 7:. PubMed
  24. Matheni C, et al. 2021. Cell J. 23:145. PubMed
  25. Yang FM, et al. 2022. Front Immunol. 13:918241. PubMed
  26. Merkt W, et al. 2021. BMC Immunol. 22:26. PubMed
  27. Rabold K, et al. 2022. Nat Commun. 13:6149. PubMed
  28. Hagel J, et al. 2021. J Immunol. 206:3073. PubMed
  29. Zhou Y, et al. 2021. Methods Mol Biol. 2388:35. PubMed
  30. Hu Z, et al. 2022. Nat Metab. 4:759. PubMed
  31. Lafôrets F, et al. 2023. iScience. 26:106514. PubMed
  32. Baughn LB, et al. 2023. Blood Cancer J. 13:84. PubMed
RRID
AB_893341 (BioLegend Cat. No. 304025)
AB_893337 (BioLegend Cat. No. 304026)

Antigen Details

Structure
Tyrosine phosphatases, type I transmembrane protein, 180-240 kD (multiple isoforms)
Distribution

Hematopoietic cells, not expressed in circulating erythrocytes or platelets

Function
TCR and BCR mediated activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Cell Type
Hematopoietic stem and progenitors, Mesenchymal Stem Cells
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
CD Molecules
Antigen References
  1. Thomas M. 1989. Annu. Rev. Immunol. 7:339.
  2. Trowbridge I, et al. 1994. Annu. Rev. Immunol.12:85.
Gene ID
5788 View all products for this Gene ID
UniProt
View information about CD45 on UniProt.org

Related FAQs

How stable is PerCP/Cy5.5 tandem as compared to PerCP alone?

PerCP/Cy5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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