Loading...

PerCP/Cyanine5.5 anti-human CD45RA Antibody

Pricing & Availability
Clone
HI100 (See other available formats)
Regulatory Status
RUO
Workshop
IV N906
Other Names
GP180, L-CA, LCA, LY5, T200, PTPRC
Isotype
Mouse IgG2b, κ
Ave. Rating
Submit a Review
Product Citations
publications
HI100_PerCPCyanine55_CD45RA_Antibody_1_060719
Human peripheral blood lymphocytes were stained with CD45RO APC and CD45RA (clone HI100) PerCP/Cyanine5.5 (left) or Mouse IgG2b, κ PerCP/Cyanine5.5 isotype control (right).
  • HI100_PerCPCyanine55_CD45RA_Antibody_1_060719
    Human peripheral blood lymphocytes were stained with CD45RO APC and CD45RA (clone HI100) PerCP/Cyanine5.5 (left) or Mouse IgG2b, κ PerCP/Cyanine5.5 isotype control (right).
See PerCP/Cyanine5.5 spectral data
Cat # Size Price Save
304121 25 tests ¥31,020
304122 100 tests ¥58,960
Description

CD45RA is a 205-220 kD single chain type I glycoprotein. It is an exon 4 splice variant of the tyrosine phosphatase CD45. The CD45RA isoform is expressed on resting/naïve T cells, medullary thymocytes, B cells and monocytes. CD45RA enhances both T cell receptor and B cell receptor signaling. CD45 non-covalently associates with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4. CD45 has also been reported to bind galectin-1. CD45 isoform expression can change in response to cytokines.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The CD45RA antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for relevant formats of this clone) include: inhibition of CD45 functions2, immunohistochemical staining of frozen tissue sections3 and formalin-fixed paraffin-embedded tissue sections4, and immunocytochemistry15,16.

Additional Product Notes

BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.

Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  2. Yamada T, et al. 2002. J. Biol. Chem. 277:28830. (WB, Block)
  3. Weninger W, et al. 2003 J. Immunol. 170:4638. (IHC-F)
  4. Imanguli MM, et al. 2009. Blood. 113:3620 (IHC-P)
  5. Roque S, et al. 2007. J. Immunol. 178:8028. (FC) PubMed
  6. Smeltz RB. 2007. J. Immunol. 178:4786. (FC) PubMed
  7. Palendira U, et al. 2008. Blood (FC) PubMed
  8. Kuttruff S, et al. 2009. Blood 113:358. (FC) PubMed
  9. Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
  10. Alanio C, et al. 2010. Blood 115:3718. (FC) PubMed
  11. Iannello A, et al. 2010. J. Immunol. 184:114. (FC) PubMed
  12. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  13. Guereau-de-Arellan M, et al. 2011. Brain. 134:3578. PubMed
  14. Canque B, et al. 2000. Blood 96:3748. (ICC)
  15. Imanguli MM, et al. 2009. Blood 13:3620. (ICC)
  16. Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
  17. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
Product Citations
  1. Ogonek J, et al. 2017. J Immunol. 10.4049/jimmunol.1601992. PubMed
  2. Maehara T, et al. 2018. Life Sci Alliance. 1:e201800050. PubMed
  3. Idorn M, et al. 2018. Oncoimmunology. 7:e1412029. PubMed
  4. Eri Yamada et al. 2018. Cell host & microbe. 23(1):110-120 . PubMed
  5. Ickrath P, et al. 2019. Biomed Rep. 10:119. PubMed
  6. Osorio FG et al. 2018. Cell reports. 25(9):2308-2316 . PubMed
  7. Liu Y, et al. 2018. JCI Insight. 3:. PubMed
  8. Ickrath P, et al. 2018. Int J Mol Med. 42:1116. PubMed
  9. Montel‐Hagen A et al. 2019. Cell stem cell. 24(3):376-389 . PubMed
  10. Qu K et al. 2017. Cancer cell. 32(1):27-41 . PubMed
  11. Rendeiro AF, et al. 2020. Nat Commun. 11:577. PubMed
  12. Freeman ZT, et al. 2020. J Clin Invest. 130:1405. PubMed
  13. Iannello A, et al. 2010. J Immunol. 184:114. PubMed
  14. Sammicheli S, et al. 2012. J Autoimmun. 38:304. PubMed
  15. Bacher P, et al. 2013. J Immunol. 190:3967. PubMed
  16. Selleri S, et al. 2011. J Allergy Clin Immunol. 127:1368. PubMed
  17. Bunse C, et al. 2013. PLoS One. 8:77925. PubMed
  18. Han L, et al. 2014. J Biol Chem. 289:25546. PubMed
  19. Eller MA, et al. 2016. J Virol. 90: 4005-4016. PubMed
  20. Aalam F, et al. 2020. PLoS Pathog. 16:e1008968. PubMed
  21. Kang L, et al. 2020. Exp Hematol Oncol. 9:11. PubMed
  22. Preglej T, et al. 2020. JCI Insight. 5(4):. PubMed
  23. Hirschberger S, et al. 2021. EMBO Mol Med. 13:e14323. PubMed
  24. Sandborn WJ, et al. 2021. Gastroenterology. 161:1853. PubMed
  25. Brandsma AM, et al. 2021. Cancer Discov. . PubMed
  26. Brandsma AM, et al. 2021. Blood Cancer Discov. 2:484. PubMed
  27. Li M, et al. 2022. Sci Rep. 12:378. PubMed
  28. Georg P, et al. 2022. Cell. 185:493. PubMed
  29. Gray DH, et al. 2022. Mol Ther Nucleic Acids. 27:505. PubMed
  30. Hasaart KAL, et al. 2022. iScience. 25:103736. PubMed
  31. Rosendahl Huber A, et al. 2022. STAR Protoc. 3:101361. PubMed
  32. Jarosch S, et al. 2022. STAR Protoc. 3:101374. PubMed
  33. Rajamanickam V, et al. 2021. Cancer Immunol Res. 9:602. PubMed
  34. de Kanter JK, et al. 2021. Cell Stem Cell. 28:1726. PubMed
  35. Zhong M, et al. 2022. Cell Death Dis. 13:671. PubMed
  36. Talvard-Balland N, et al. 2021. J Invest Dermatol. 141:193. PubMed
  37. Jarosch S, et al. 2021. Cell Rep Methods. 1:100104. PubMed
  38. Rasmussen TA, et al. 2022. Cell Rep Med. 3:100766. PubMed
  39. Hagel J, et al. 2021. J Immunol. 206:3073. PubMed
  40. Gkika E, et al. 2023. NPJ Precis Oncol. 7:24. PubMed
RRID
AB_893358 (BioLegend Cat. No. 304121)
AB_893357 (BioLegend Cat. No. 304122)

Antigen Details

Structure
Tyrosine phosphatases, type I transmembrane (exon 4 splicing of CD45 gene), 205-220 kD
Distribution

B cells, naïve T cells, monocytes

Function
Enhances TCR and BCR signaling
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Cell Type
B cells, Monocytes, T cells, Tregs
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Thomas M. 1989. Annu. Rev. Immunol. 7:339.
2. Trowbridge I, et al. 1994. Annu. Rev. Immunol.12:85.

Gene ID
5788 View all products for this Gene ID
UniProt
View information about CD45RA on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 5    Revision Date: 06/07/2019

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account