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PE/Cyanine5 anti-human CD19 Antibody

Pricing & Availability
Clone
HIB19 (See other available formats)
Regulatory Status
RUO
Workshop
V CD19.11
Other Names
B4
Isotype
Mouse IgG1, κ
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Product Citations
publications
HIB19
Human peripheral blood lymphocytes stained with HIB19 PE/Cyanine5
  • HIB19
    Human peripheral blood lymphocytes stained with HIB19 PE/Cyanine5
See PE/Cyanine5 spectral data
Cat # Size Price Quantity Check Availability Save
302209 25 tests 93€
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302210 100 tests 208€
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Description

CD19 is a 95 kD type I transmembrane glycoprotein also known as B4. It is a member of the immunoglobulin superfamily expressed on B-cells (from pro-B to blastoid B cells, absent on plasma cells) and follicular dendritic cells. CD19 is involved in B cell development, activation, and differentiation. CD19 forms a complex with CD21 (CR2) and CD81 (TAPA-1), and functions as a BCR co-receptor.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine5 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections8 and blocking of B cell proliferation. Clone HIB19 is not recommended for formalin-fixed paraffin-embedded sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 302267 & 302268).

 

Clone HIB19 partially blocks anti-human CD19 clones 4G7 and SJ25C1 staining based on in-house testing

Additional Product Notes

BioLegend is in the process of converting the name PE/Cy5 to PE/Cyanine5. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine5 products. Please contact Technical Service if you have any questions.

Application References
  1. Schlossman S, et al. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  3. Bradbury L, et al. 1993. J. Immunol. 151:2915.
  4. Joseph A, et al. 2010. J. Virol. 84:6645. PubMed
  5. Wang X, et al. 2010. Haematologica. 95:884. (FC) PubMed
  6. Walker JD, et al. 2009. J. Immunol. 182:1548. (Block) PubMed
  7. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  8. Hansen A, et al. 2002. Arthritis Rheum. 46:2160. (IHC)
  9. Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
  10. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
Product Citations
  1. Yuan Z, et al. 2018. Emerg Microbes Infect. 7:59. PubMed
  2. Bill M, et al. 2018. J Cell Mol Med. 22:2311. PubMed
  3. Sibener LV et al. 2018. Cell. 174(3):672-687 . PubMed
  4. Lagou V et al. 2018. Cell reports. 25(3):798-810 . PubMed
  5. Rodriguez‐Meira A et al. 2019. Molecular cell. 73(6):1292-1305 . PubMed
  6. Cassetta L et al. 2019. Cancer Cell. 35(4):588-602 . PubMed
  7. Jung J, et al. 2019. Cell Rep. 26:1906. PubMed
  8. Del Alcazar D, et al. 2019. Cell Rep. 28:3047. PubMed
  9. Ratliff ML, et al. 2019. J Autoimmun. 96:158. PubMed
  10. Franco LM, et al. 2019. J Exp Med. 216:384. PubMed
  11. van den Hoogen P, et al. 2020. PLoS One. 14:e0227283. PubMed
  12. Gupta R, et al. 2020. Cell Stem Cell. 26:527. PubMed
  13. Di Genua C, et al. 2020. Cancer Cell. 37(5):690-704.e8.. PubMed
  14. Labuhn M, et al. 2020. Cancer Cell. 36(2):123-138. PubMed
  15. Grifoni A, et al. 2020. Cell. 181(7):1489-1501.e15.. PubMed
  16. Wang L, et al. 2014. Proc Natl Acad Sci U S A. 111:3146. PubMed
  17. Boisson B, et al. 2015. J Exp Med. 212:939. PubMed
  18. Fletcher H, et al. 2016. Nat Commun. 7: 11290. PubMed
  19. Nilsson A, et al. 2016. PLoS One. 11: 0158369. PubMed
  20. Demers K, et al. 2016. PLoS Pathog. 12: 1005805. PubMed
  21. Yuan Z, et al. 2016. J Virol. 90: 7728 - 7739. PubMed
  22. Rydyznski Moderbacher C, et al. 2020. Cell. 183(4):996-1012.e19. PubMed
  23. Wu VH, et al. 2020. JCI Insight. 5:00. PubMed
  24. Pan YG, et al. 2021. Immunity. 54(6):1245-1256.e5. PubMed
  25. Ziegler CGK, et al. 2021. Cell. 184(18):4713-4733.e22. PubMed
  26. Alsaleh G, et al. 2020. Elife. 9: . PubMed
  27. Geuijen C, et al. 2021. Nat Commun. 12:4445. PubMed
  28. Lima NS, et al. 2022. Nat Commun. 13:7733. PubMed
RRID
AB_314239 (BioLegend Cat. No. 302209)
AB_314240 (BioLegend Cat. No. 302210)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, 95 kD
Distribution

B lineage (except plasma cells), follicular dendritic cells

Function
B cell activation and differentiation
Ligand/Receptor
Forms complex with CD21 (CR2) and CD81 (TAPA-1), BCR coreceptor
Cell Type
B cells, Dendritic cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Tedder T, et al. 1994. Immunol. Today 15:437.
2. Bradbury L, et al. 1993. J. Immunol. 151:2915.

Gene ID
930 View all products for this Gene ID
UniProt
View information about CD19 on UniProt.org
Go To Top Version: 2    Revision Date: 12/18/2013

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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