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PE/Cyanine7 anti-human CD69 Antibody

Pricing & Availability
Clone
FN50 (See other available formats)
Regulatory Status
RUO
Workshop
IV A91
Other Names
Very Early Activation Antigen (VEA), Activation inducer molecule (AIM)
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
FN50_PECy7_070207
PMA+ionomycin activated human peripheral blood lymphocytes stained with FN50 PE/Cyanine7
  • FN50_PECy7_070207
    PMA+ionomycin activated human peripheral blood lymphocytes stained with FN50 PE/Cyanine7
See PE/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability Save
310911 25 tests 115€
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310912 100 tests 234€
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Description

CD69 is a 27-33 kD type II transmembrane protein also known as activation inducer molecule (AIM), very early activation antigen (VEA), and MLR3. It is a member of the C-type lectin family, expressed as a disulfide-linked homodimer. Other members of this receptor family include NKG2, NKR-P1 CD94, and Ly49. CD69 is transiently expressed on activated leukocytes including T cells, thymocytes, B cells, NK cells, neutrophils, and eosinophils. CD69 is constitutively expressed by a subset of medullary mature thymocytes, platelets, mantle B cells, and certain CD4+ T cells in germinal centers of normal lymph nodes. CD69 is involved in early events of lymphocyte, monocyte, and platelet activation, and has a functional role in redirected lysis mediated by activated NK cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections2, immunofluorescence microscopy3, and spatial biology (IBEX)8,9.

Additional Product Notes
BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References
  1. Knapp WB, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  2. Sakkas LI, et al. 1998. Clin. and Diag. Lab. Immunol. 5:430. (IHC)
  3. Kim JR, et al. 2005. BMC Immunol. 6:3. (IF)
  4. Verjans GM, et al. 2007. P. Natl. Acad. Sci. USA 104:3496.
  5. Lu H, et al. 2009. Toxicol Sci. 112:363. (FC) PubMed
  6. Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
  7. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  8. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  9. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Maritz MF, et al. 2018. PLoS One. 13:e0196043. PubMed
  2. Horwitz JA, et al. 2017. Cell. 170:637. PubMed
  3. Ray RM, et al. 2019. Sci Rep. 9:4334. PubMed
  4. Ormonde JVS, et al. 2018. J Immunol. 201:3431. PubMed
  5. Pham TNQ, et al. 2020. Cell Reports. 29(9):2770-2782.e5.. PubMed
  6. Lu H, et al. 2009. Toxicol Sci. 112:363. PubMed
  7. Smolders J, et al. 2010. PLoS One. 5:e15235. PubMed
  8. Presicce P, et al. 2011. PLoS One. 6:e28118. PubMed
  9. Assen S, et al. 2011. Clin Immunol. 141:161. PubMed
  10. Knippenberg S, et al. 2011. J Neuroimmunol. 28:239. PubMed
  11. Ophir M, et al. 2013. J Cell Biol. 203:1021. PubMed
  12. Hall J, et al. 2014. Brain Behav Immun. 36:156. PubMed
  13. Ribot J, et al. 2014. J Immunol. 192:2237. PubMed
  14. Phadnis-Moghe A, et al. 2015. Toxicol Sci. 144:39. PubMed
  15. Inui M, et al. 2015. Int Immunol. 27: 345 - 355. PubMed
  16. Jürchott K, et al. 2016. PLoS One. 11: 0150812. PubMed
  17. Pachnio A, et al. 2016. PLoS Pathog. 12: 1005832. PubMed
  18. Gullà A, et al. 2021. Cancer Discov. 2:468. PubMed
  19. Gullà A, et al. 2021. Cancer Discov. . PubMed
  20. Mudd PA, et al. 2022. Cell. 185:603. PubMed
  21. Valestrand L, et al. 2022. Am J Pathol. . PubMed
  22. Lee-Chang C, et al. 2021. J Exp Med. 218:. PubMed
  23. Larsen SE, et al. 2021. Sci Rep. 11:9040. PubMed
  24. Lozano-Ojalvo D, et al. 2021. Cell Rep. 36:109570. PubMed
  25. Balachandran H, et al. 2022. Cell Rep. 38:110345. PubMed
  26. Wang H, et al. 2022. Front Immunol. 13:852436. PubMed
  27. Vadrevu KM, et al. 2022. Sci Rep. 12:12038. PubMed
  28. Csomós K, et al. 2022. Nat Immunol. 23:1256. PubMed
  29. Busek M, et al. 2023. Lab Chip. 23:591. PubMed
RRID
AB_314846 (BioLegend Cat. No. 310911)
AB_314847 (BioLegend Cat. No. 310912)

Antigen Details

Structure
C-type lectin, type II glycoprotein, 28/32 kD
Distribution

Activated T cells, B cells, NK cells, granulocytes, thymocytes, platelets, Langerhans cells

Function
Lymphocyte, monocyte, and platelet activation, NK cell killing
Cell Type
B cells, Granulocytes, Langerhans cells, NK cells, Platelets, T cells, Thymocytes, Tregs
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
2. Testi R, et al. 1994. Immunol. Today 15:479.

Gene ID
969 View all products for this Gene ID
UniProt
View information about CD69 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/30/2012

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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