- Clone
- 145-2C11 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- CD3ε, T3, CD3
- Isotype
- Armenian Hamster IgG
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![145-2C11_Pure_021606 145-2C11_Pure_021606](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/145-2C11_Pure_021606.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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C57BL/6 mouse splenocytes were stained with purified CD3e (clone 145-2C11) (filled histogram) or Armenian hamster IgG isotype control (open histogram), followed by anti-Armenian hamster IgG FITC. -
C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/mL of purified anti-mouse CD3ε (clone 145-2C11) and 10 µg/mL of Alexa Fluor® 488 anti-mouse/human CD45R/B220 (clone RA3-6B2) (green) overnight at 4°C, followed by 2.5 µg/mL of Alexa Fluor® 594 Goat anti-hamster (Armenian) IgG (clone Poly4055) (red) for two hours at room temperature. The image was captured by 10X objective. -
Fresh, frozen mouse spleen was stained with purified CD3ε clone 145-2C11 conjugated and detected with a Cy3 CODEX™ oligonucleotide duplex (red). Samples were counterstained with B220 A647 (green). Data generated at Akoya Biosciences, Inc. using the CODEX™ technology.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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100301 | 50 µg | 20€ | ||||
100302 | 500 µg | 58€ |
CD3ε is a 20 kD transmembrane protein, also known as CD3 or T3. It is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3ε forms a TCR complex by associating with the CD3δ, γ and ζ chains, as well as the TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Armenian Hamster
- Immunogen
- H-2Kb-specific mouse cytotoxic T lymphocyte clone BM10-37
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The CD3ε antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
IHC-F - Verified
IP, Activ, Block, WB, ICC - Reported in the literature, not verified in house - Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. For immunohistochemical staining on frozen tissue sections, the suggested use of this reagent is 5.0 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Clone 145-2C11 is useful for in vitro blocking of target-specific CTL-mediated cell lysis1, as well as T cell activation assays, inducing proliferation and cytokine production1,2,7,12,16. It also induces apoptosis in immature thymocytes32, and in vivo T cell depletion8-10. Additional reported applications (for relevant formats of this clone) include: immunoprecipitation1, immunohistochemical staining14,15 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, Western blotting4, complement-mediated cytotoxicity6, in vitro and in vivo stimulation of T cells1,2,7,12,16, immunofluorescent staining5, and in vivo T cell depletion8-10. The 145-2C11 antibody has been reported to block the binding of 17A2 antibody to CD3 epsilon-specific T cells11. Clone 145-2C11 is not recommended for formalin-fixed paraffin embedded sections. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 100314). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 100340) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
- Application References
-
- Leo O, et al. 1987. P. Natl. Acad. Sci. USA 84:1374. (IP, Activ, Block)
- Kruisbeek AM, et al. 1991. In Current Protocols in Immunology. 3.12.1. (Activ)
- Duke RC, et al. 1995. Current Protocols in Immunology. 3.17.1.
- Salvadori S, et al. 1994. J. Immunol. 153:5176. (WB)
- Payer E, et al. 1991. J. Immunol. 146:2536. (IF)
- Jacobs H, et al. 1994. Eur. J. Immunol. 24:934. (CMCD)
- Vossen ACTM, et al. 1995. Eur. J. Immunol. 25:1492. (Activ)
- Henrickson M, et al. 1995. Transplantation 60:828. (Deplete)
- Kinnaert P, et al. 1996. Transpl. Int. 9:386. (Deplete)
- Han WR, et al. 1999. Transpl. Immunol. 7:207. (Deplete)
- Miescher GC, et al. 1989. Immunol. Lett. 23:113. (Block)
- Terrazas LI, et al. 2005. Intl. J. Parasitology. 35:1349. (Activ)
- Ko SY, et al. 2005. J. Immunol. 175:3309.
- Podd BS, et al. 2006. J. Immunol. 176:6532. (IHC-F)
- Tilley SL, et al. 2007. J. Immunol. 178:3208. (IHC-F)
- Wang W, et al. 2007. J. Immunol. 178:4885. (Activ)
- Xiao S, et al. 2007. J. Exp. Med. 204:1691.
- Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245. (FC) PubMed.
- Curtsinger JM, et al.2005. J. Immunol. 175:4392. PubMed
- Guo Y, et al. 2008. Blood 112:480. PubMed
- Kenna TJ, et al. 2008. Blood 111:2091.
- Perchonock CE, et al. 2007. J. Immunol. 179:1768. PubMed
- Perchonock GE, et al. 2006. Mol. Cell. Biol. 26:6005. PubMed
- Kanaya T, et al. 2008. Am. J. Physiol. Gastrointest. Liver Physiol. 295:G273. PubMed
- de Koning BA, et al. 2006. Int. Immunol. 18:941. PubMed
- Schulteis RD, et al. 2008. Blood 295:G273. PubMed
- Qi Q, et al. 2009. Blood 114:564. PubMed
- Helmersson S, et al. 2013. Am J Pathol. 9440:123. Pubmed
- Wu S, et al. 2014. Clin Vaccine Immunol. 21:156. PubMed
- Yan J, et al. 2014. Vaccine. 32:2833. PubMed
- Guiterrez DA, et al. 2014. Diaebetes. 63:3827. PubMed
- Shi YF, et al. 1991. J Immunol. 146:3340. (Apop)
- Product Citations
-
- RRID
-
AB_312666 (BioLegend Cat. No. 100301)
AB_312667 (BioLegend Cat. No. 100302)
Antigen Details
- Structure
- Ig superfamily, forms CD3/TCR complex with CD3δ, γ and ζ subunits and TCR (α/β and γ/δ), 20 kD
- Distribution
-
Thymocytes (differentiation dependent), mature T cells, NK-T cells
- Function
- TCR signal transduction, T cell activation, antigen recognition
- Ligand/Receptor
- Peptide antigen/MHC-complex
- Cell Type
- NKT cells, T cells, Thymocytes, Tregs
- Biology Area
- Immunology
- Molecular Family
- CD Molecules, TCRs
- Antigen References
-
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Davis MM. 1990. Annu. Rev. Biochem. 59:475.
3. Weiss A, et al. 1994. Cell 76:263. - Gene ID
- 12501 View all products for this Gene ID
- Specificity (DOES NOT SHOW ON TDS):
- CD3epsilon
- Specificity Alt (DOES NOT SHOW ON TDS):
- CD3ε
- App Abbreviation (DOES NOT SHOW ON TDS):
- FC,IHC-F,IP,Activ,Block,WB,ICC
- UniProt
- View information about CD3epsilon on UniProt.org
Related Pages & Pathways
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Other Formats
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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APC anti-mouse CD3ε
C57BL/6 mouse splenocytes were stained with CD3e (clone 145-... -
Biotin anti-mouse CD3ε
C57BL/6 mouse splenocytes were stained with biotinylated CD3... C57BL/6 mouse frozen spleen section was fixed with 4% parafo... -
FITC anti-mouse CD3ε
C57BL/6 mouse splenocytes were stained with CD3e (clone 145-... -
PE anti-mouse CD3ε
C57BL/6 mouse splenocytes were stained with CD3e (clone 145-... C57BL/6 mouse splenocytes were stained with CD3e (clone 145-... -
PE/Cyanine5 anti-mouse CD3ε
C57BL/6 mouse splenocytes were stained with CD3e (clone 145-... -
Purified anti-mouse CD3ε
C57BL/6 mouse splenocytes were stained with purified CD3e (c... C57BL/6 mouse frozen spleen section was fixed with 4% parafo... Fresh, frozen mouse spleen was stained with purified CD3ε cl... -
PE/Cyanine7 anti-mouse CD3ε
C57BL/6 mouse splenocytes were stained with CD3e (clone 145-... -
Alexa Fluor® 488 anti-mouse CD3ε
C57BL/6 mouse splenocytes were stained with CD3e (clone 145-... -
Alexa Fluor® 647 anti-mouse CD3ε
C57BL/6 mouse splenocytes were stained with CD3e (clone 145-... C57BL/6 mouse frozen spleen section was fixed with 4% parafo... Paraformaldehyde-fixed (4%), 500 μm-thick mouse spleen secti... -
PerCP anti-mouse CD3ε
C57BL/6 mouse splenocytes were stained with CD3e (clone 145-... -
PerCP/Cyanine5.5 anti-mouse CD3ε
C57BL/6 splenocytes were stained with CD45R/B220 APC and CD3... -
Purified anti-mouse CD3ε (Maxpar® Ready)
Mouse splenocytes stained with 152Sm anti-CD3e (145-2C11) an... -
APC/Cyanine7 anti-mouse CD3ε
C57BL/6 mouse splenocytes were stained with CD3e (clone 145-... -
Pacific Blue™ anti-mouse CD3ε
C57BL/6 mouse splenocytes were stained with CD3e (clone 145-... -
Brilliant Violet 421™ anti-mouse CD3ε
C57BL/6 mouse splenocytes were stained with CD19 FITC and CD... C57BL/6 mouse splenocytes were fixed with 2% paraformaldehyd... -
Ultra-LEAF™ Purified anti-mouse CD3ε
C57BL/6 mouse splenocytes were stained with LEAF™ purified C... -
PE/Dazzle™ 594 anti-mouse CD3ε
C57BL/6 mouse splenocytes were stained with CD3ε (cl... -
Brilliant Violet 510™ anti-mouse CD3ε
C57BL/6 mouse splenocytes were stained with CD3ε (cl... -
Brilliant Violet 605™ anti-mouse CD3ε
C57BL/6 mouse splenocytes were stained with CD3ε (cl... -
Brilliant Violet 711™ anti-mouse CD3ε
C57BL/6 mouse splenocytes were stained with CD3ε (cl... -
Brilliant Violet 785™ anti-mouse CD3ε
C57BL/6 mouse splenocytes were stained with CD3ε (cl... -
APC/Fire™ 750 anti-mouse CD3ε
C57BL/6 splenocytes were stained with CD19 FITC and CD3&epsi... -
GoInVivo™ Purified anti-mouse CD3ε
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Spark YG™ 593 anti-mouse CD3
C57BL/6 splenocytes were stained with anti-mouse CD45R/B220 ... -
PerCP/Fire™ 780 anti-mouse CD3ε
C57BL/6 mouse splenocytes were stained with anti-mouse CD19... -
Brilliant Violet 650™ anti-mouse CD3ε
C57BL/6 mouse splenocytes were stained with anti-mouse/human...
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