Loading...

FITC anti-mouse CD3ε Antibody

Pricing & Availability
Clone
145-2C11 (See other available formats)
Regulatory Status
RUO
Other Names
CD3ε, T3, CD3
Isotype
Armenian Hamster IgG
Ave. Rating
Submit a Review
Product Citations
publications
145-2C11_FITC_021606
C57BL/6 mouse splenocytes were stained with CD3e (clone 145-2C11) FITC and CD127 (clone SB/199) PE.
  • 145-2C11_FITC_021606
    C57BL/6 mouse splenocytes were stained with CD3e (clone 145-2C11) FITC and CD127 (clone SB/199) PE.
Compare all formats See FITC spectral data
Cat # Size Price Quantity Check Availability Save
100305 50 µg 24€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
100306 500 µg 80€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD3ε is a 20 kD transmembrane protein, also known as CD3 or T3. It is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3ε forms a TCR complex by associating with the CD3δ, γ and ζ chains, as well as the TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
H-2Kb-specific mouse cytotoxic T lymphocyte clone BM10-37
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Clone 145-2C11 is useful for in vitro blocking of target-specific CTL-mediated cell lysis1, as well as T cell activation assays, inducing proliferation and cytokine production1,2,7,12,16. It also induces apoptosis in immature thymocytes32,  and in vivo T cell depletion8-10. Additional reported applications (for relevant formats of this clone) include: immunoprecipitation1, immunohistochemical staining14,15 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, Western blotting4, complement-mediated cytotoxicity6, in vitro and in vivo stimulation of T cells1,2,7,12,16, immunofluorescent staining5, and in vivo T cell depletion8-10. The 145-2C11 antibody has been reported to block the binding of 17A2 antibody to CD3 epsilon-specific T cells11. Clone 145-2C11 is not recommended for formalin-fixed paraffin embedded sections. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 100314). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 100340) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Application References
  1. Leo O, et al. 1987. P. Natl. Acad. Sci. USA 84:1374. (IP, Activ, Block)
  2. Kruisbeek AM, et al. 1991. In Current Protocols in Immunology. 3.12.1. (Activ)
  3. Duke RC, et al. 1995. Current Protocols in Immunology. 3.17.1.
  4. Salvadori S, et al. 1994. J. Immunol. 153:5176. (WB)
  5. Payer E, et al. 1991. J. Immunol. 146:2536. (IF)
  6. Jacobs H, et al. 1994. Eur. J. Immunol. 24:934. (CMCD)
  7. Vossen ACTM, et al. 1995. Eur. J. Immunol. 25:1492. (Activ)
  8. Henrickson M, et al. 1995. Transplantation 60:828. (Deplete)
  9. Kinnaert P, et al. 1996. Transpl. Int. 9:386. (Deplete)
  10. Han WR, et al. 1999. Transpl. Immunol. 7:207. (Deplete)
  11. Miescher GC, et al. 1989. Immunol. Lett. 23:113. (Block)
  12. Terrazas LI, et al. 2005. Intl. J. Parasitology. 35:1349. (Activ)
  13. Ko SY, et al. 2005. J. Immunol. 175:3309.
  14. Podd BS, et al. 2006. J. Immunol. 176:6532. (IHC-F)
  15. Tilley SL, et al. 2007. J. Immunol. 178:3208. (IHC-F)
  16. Wang W, et al. 2007. J. Immunol. 178:4885. (Activ)
  17. Xiao S, et al. 2007. J. Exp. Med. 204:1691.
  18. Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245. (FC) PubMed.
  19. Curtsinger JM, et al.2005. J. Immunol. 175:4392. PubMed
  20. Guo Y, et al. 2008. Blood 112:480. PubMed
  21. Kenna TJ, et al. 2008. Blood 111:2091.
  22. Perchonock CE, et al. 2007. J. Immunol. 179:1768. PubMed
  23. Perchonock GE, et al. 2006. Mol. Cell. Biol. 26:6005. PubMed
  24. Kanaya T, et al. 2008. Am. J. Physiol. Gastrointest. Liver Physiol. 295:G273. PubMed
  25. de Koning BA, et al. 2006. Int. Immunol. 18:941. PubMed
  26. Schulteis RD, et al. 2008. Blood 295:G273. PubMed
  27. Qi Q, et al. 2009. Blood 114:564. PubMed
  28. Helmersson S, et al. 2013. Am J Pathol. 9440:123. Pubmed
  29. Wu S, et al. 2014. Clin Vaccine Immunol. 21:156. PubMed
  30. Yan J, et al. 2014. Vaccine. 32:2833. PubMed
  31. Guiterrez DA, et al. 2014. Diaebetes. 63:3827. PubMed
  32. Shi YF, et al. 1991. J Immunol. 146:3340. (Apop)
Product Citations
  1. Russler-Germain EV, et al. 2021. Immunity. 54:2547. PubMed
  2. Li DD, et al. 2022. J Cell Mol Med. 26:2438. PubMed
  3. Jou E, et al. 2022. Sci Immunol. 7:eabn0175. PubMed
  4. Zhao X, et al. 2022. STAR Protoc. 3:101859. PubMed
  5. Yang L, et al. 2023. Acta Pharmacol Sin. :. PubMed
  6. Lu T, et al. 2023. Cancer Immunol Immunother. :. PubMed
  7. Smith LA, et al. 2023. Front Oncol. 12:1031174. PubMed
  8. Murakata Y, et al. 2023. Front Cardiovasc Med. 9:1075358. PubMed
  9. Jerome JR, et al. 2023. Int J Mol Sci. 24: . PubMed
  10. Drijvers JM, et al. 2021. Cancer Immunol Res. 9:184. PubMed
  11. Chei S, et al. 2020. Front Nutr. 7:583186. PubMed
  12. Bertrand F, et al. 2017. Nat Commun. 8:2256. PubMed
  13. Katsumura KR, et al. 2018. Proc Natl Acad Sci U S A. 115:E10109. PubMed
  14. Hou X, et al. 2020. Cell Reports. 28(1):172-189.e7.. PubMed
  15. Tomida S, et al. 2019. Sci Rep. 9:10751. PubMed
  16. Glorieux C, et al. 2022. J Adv Res. 40:109. PubMed
  17. Wu R, et al. 2022. Nat Immunol. 23:1536. PubMed
  18. Lu C, et al. 2023. Antiviral Res. 212:105556. PubMed
  19. Wang JX, et al. 2023. JCI Insight. 8:. PubMed
  20. Wu Y, et al. 2023. Nat Cancer. 4:382. PubMed
  21. Brunner SM, et al. 2023. Int J Mol Sci. 24:. PubMed
  22. Senatus L, et al. 2023. Commun Biol. 6:280. PubMed
  23. Stitzlein LM, et al. 2023. Front Neurol. 14:1112207. PubMed
  24. van Os BW, et al. 2023. Front Cardiovasc Med. 10:1171764. PubMed
  25. Ye S, et al. 2023. Front Oncol. 13:1202750. PubMed
  26. Zhou J, et al. 2021. Int J Med Sci. 18:3516. PubMed
  27. Lentucci C, et al. 2017. J Biol Chem. 292:2754-2772. PubMed
  28. Schulteis R, et al. 2008. Blood. 112:4905. PubMed
  29. Wang L, et al. 2019. Cell Rep. 29:1848. PubMed
  30. Groza D, et al. 2018. Oncoimmunology. 7:e1424676. PubMed
  31. Hewitson JP, et al. 2020. J Immunol. 204:2949. PubMed
  32. Johnson KD, et al. 2022. Blood Adv. 6:1464. PubMed
  33. Fornari T, et al. 2015. PLoS One. 10: 0142688. PubMed
  34. Koning B, et al. 2006. Int Immunol. 1.403472222. PubMed
  35. Waide ML, et al. 2020. Cell Rep. 33:108503. PubMed
  36. Kremenovic M, et al. 2022. J Immunother Cancer. 10:. PubMed
  37. Tian H, et al. 2022. Cancer Sci. 113:875. PubMed
  38. Lou F, et al. 2020. STAR Protoc. 1:100115. PubMed
  39. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  40. Gruber T, et al. 2020. JCI Insight. 5:00. PubMed
  41. Allegrezza M, et al. 2016. Cancer Res . 76: 6253 - 6265. PubMed
  42. Yang W, et al. 2020. Nat Commun. 3.553472222. PubMed
  43. Isoda T et al. 2017. Cell. 171(1):103-119 . PubMed
  44. Hoffman D, et al. 2021. Immunity. 54:2712. PubMed
  45. Patnode M, et al. 2013. Glycobiology. 23:381. PubMed
  46. Bartleson JM, et al. 2020. Nat Immunol. 1384:21. PubMed
  47. Mann M, et al. 2018. Cell Rep. 25:2992. PubMed
  48. Hung PJ, et al. 2018. Mol Cell. 71:332. PubMed
  49. Uchida A, et al. 2021. J Inflamm Res. 14:3089. PubMed
  50. Sato Y, et al. 2021. J Clin Invest. Online ahead of print. PubMed
  51. Yi M, et al. 2021. J Hematol Oncol. 14:27. PubMed
  52. Zhang X, et al. 2020. J Immunol. 205:1743. PubMed
  53. Peng L, et al. 2020. Transl Lung Cancer Res. 0.892361111. PubMed
  54. Best SA, et al. 2018. Cell Metab. 27:935. PubMed
  55. Pereira JL, et al. 2021. Transl Oncol. 14:101125. PubMed
  56. Li H, et al. 2022. Theranostics. 12:6422. PubMed
  57. Collin R, et al. 2014. J Immunol. 193:3503. PubMed
  58. Yin Q, et al. 2015. PLoS One. 10: 0137808. PubMed
  59. Ma Y, et al. 2015. J Immunol. 195: 3769 - 3780. PubMed
  60. Matsumura K, et al. 2016. J Immunol. 197: 3233 - 3244. PubMed
  61. Emgård J, et al. 2018. Immunity. 143:419. PubMed
  62. Lin JR et al. 2018. eLife. 7 pii: e31657. PubMed
  63. Shimokawa C et al. 2017. Immunity. 46(5):863-874 . PubMed
  64. Qi X, et al. 2019. Nat Med. 25:1225. PubMed
  65. Jeong SH, et al. 2021. Nat Commun. 12:4405. PubMed
  66. Huang Y, et al. 2020. FASEB J. 34:1768. PubMed
  67. Kawakami R, et al. 2021. Immunity. 54(5):947-961.e8. PubMed
  68. Burrack K, et al. 2015. PLoS Pathog. 11: e1005191. PubMed
  69. Skeate JG, et al. 2018. PLoS One. 13:e0191311. PubMed
  70. He L, et al. 2019. iScience. 0.790972222. PubMed
  71. Cheng HW, et al. 2019. Nat Commun. 10:1739. PubMed
  72. Cong J et al. 2018. Cell metabolism. 28(2):243-255 . PubMed
  73. Russler-Germain EV, et al. 2021. Elife. 10:. PubMed
  74. Pardy RD, et al. 2021. Nat Commun. 12:4051. PubMed
  75. Yang H, et al. 2022. Nutrients. 14:. PubMed
  76. Kondratieva T, et al. 2020. PLoS One. 15:e0239668. PubMed
  77. Miki Y, et al. 2016. J Biol Chem. 291: 15588 - 15601. PubMed
  78. Tang CC, et al. 2021. eLife. 10:00. PubMed
  79. Gong Y, et al. 2020. Cell Metabolism. 33(1):51-64.e9. PubMed
  80. He Y, et al. 2021. Cell Metabolism. 33(5):988-1000.e7. PubMed
  81. Souza COS, et al. 2021. iScience. 24(6):102548. PubMed
  82. Derecka M, et al. 2020. Nat Immunol. 261:21. PubMed
  83. Knolle MD et al. 2018. Frontiers in immunology. 1.925 . PubMed
  84. Doonan J, et al. 2019. Nat Commun. 10:1554. PubMed
  85. Srivastava S, et al. 2019. Cancer Cell. 35:489. PubMed
  86. Harnett MM, et al. 2022. Front Immunol. 13:953053. PubMed
  87. Zeng Q, et al. 2022. Front Immunol. 13:740805. PubMed
  88. Stotesbury C, et al. 2020. J Immunol. 204:1582. PubMed
  89. Ringel AE, et al. 2020. Cell. 183(7):1848-1866.e26. PubMed
  90. Yi X, et al. 2020. Sci Adv. 6:eaba3546. PubMed
  91. Burrack K, et al. 2015. J Immunol. 194:678. PubMed
  92. Siamishi I, et al. 2020. Cell Reports. 31(11):107756. PubMed
  93. Wanner-Seleznik GM, et al. 2020. Immunohorizons. 0.644444444. PubMed
  94. Deerhake ME, et al. 2021. Immunity. 54(3):484-498.e8. PubMed
  95. Kim SH, et al. 2021. Cell Reports. 35(2):108995. PubMed
  96. Xiang W, et al. 2018. Nat Commun. 9:2574. PubMed
  97. Kacherovsky N, et al. 2019. Nat Biomed Eng. 0.66875. PubMed
  98. Murakami K, et al. 2021. Cell Reports. 34(1):108579. PubMed
  99. Ni J, et al. 2020. Immunity. 52(6):1075-1087.e8. PubMed
  100. Bian G, et al. 2020. British Journal of Pharmacology. 178(3):636-653. PubMed
  101. Galle-Treger L, et al. 2016. Nat Commun. 7:13202. PubMed
  102. Sendler M, et al. 2020. Gastroenterology. 158:253. PubMed
  103. Denny JE, et al. 2019. Sci Rep. 2.786111111. PubMed
  104. Kim MY, et al. 2017. Plant Biotechnol J. 1.729166667. PubMed
  105. Xu Q, et al. 2021. Theranostics. 11:1937. PubMed
  106. Zhang P, et al. 2022. Nat Commun. 13:1588. PubMed
  107. Xu M, et al. 2013. J Immunol. 190:5436. PubMed
  108. Huang J, et al. 2014. J Immunol. 192:1972. PubMed
  109. Vacca F, et al. 2020. eLife. 9:e54017.. PubMed
  110. Labiano S, et al. 2017. Oncoimmunology. 6:e1283468. PubMed
  111. Engblom C, et al. 2017. Science. 358:6367. PubMed
  112. Baomei Wang et al. 2019. Cell reports. 26(6):1614-1626 . PubMed
  113. Lind L, et al. 2021. J Neurovirol. 27:145. PubMed
  114. Agrawal M, et al. 2022. Blood. 140:1094. PubMed
  115. Zhao X, et al. 2022. iScience. 25:104690. PubMed
  116. Soria-Castro R, et al. 2020. Sci Rep. 10:17802. PubMed
  117. Kang YH, et al. 2019. Nat Commun. 10:912. PubMed
  118. Eisemann T, et al. 2019. Front Oncol. 9:187. PubMed
  119. Yue W, et al. 2019. Nat Commun. 10:2025. PubMed
  120. Oliveira AC et al. 2017. eLife. 6 pii: e30883. PubMed
  121. Sánchez-del-Campo L, et al. 2021. J Exp Clin Cancer Res. 40:117. PubMed
  122. Casselli T, et al. 2021. PLoS Pathog. 17:e1009256. PubMed
  123. Okada R, et al. 2021. EBioMedicine. 67:103345. PubMed
  124. Luo L, et al. 2021. J Neuroinflammation. 18:27. PubMed
  125. Damgaard RB et al. 2016. Cell. 166(5):1215-1230 . PubMed
  126. Baglaenko Y, et al. 2016. PLoS One. 11: 0150515. PubMed
  127. Wang J, et al. 2019. Aging (Albany NY). 11:3463. PubMed
  128. Lin JC, et al. 2019. Nat Commun. 10:1057. PubMed
  129. Liu Y, et al. 2018. Cancer Cell. 33:480. PubMed
  130. La Salvia S, et al. 2020. Am J Physiol Renal Physiol. 319:F868. PubMed
  131. Fitzpatrick Z, et al. 2020. Nature. 587:472. PubMed
  132. Okuyama Y, et al. 2020. FASEB J. 540:34. PubMed
  133. Sparber F, et al. 2019. Cell Host Microbe. 25:389. PubMed
  134. Zegarra‐Ruiz DF et al. 2018. Cell host & microbe. 25(1):113-127 . PubMed
  135. Oh J, et al. 2021. Clin Cancer Res. 27:4781. PubMed
  136. Harding CL, et al. 2020. Front Cell Infect Microbiol. 10:328. PubMed
  137. Xiao Z, et al. 2022. Mater Today Bio. 15:100297. PubMed
  138. Ma C, et al. 2021. Mol Metab. 47:101170. PubMed
  139. Srivastava S, et al. 2020. Cancer Cell. 39(2):193-208.e10. PubMed
  140. Sheng J, et al. 2021. eLife. 10:00. PubMed
  141. Nakagawa Y, et al. 2015. Immunol Lett. 167: 72-86. PubMed
  142. Ikeda T, et al. 2014. PLoS One. 9:115198. PubMed
  143. Burton OT, et al. 2018. Clin Exp Allergy. 48:288. PubMed
  144. Tippimanchai DD, et al. 2018. Oncoimmunology. 7:e1438105. PubMed
  145. Schofield L, et al. 2017. BMC Med. 10.1186/s12916-017-0883-8. PubMed
  146. Zhou X, et al. 2021. Nutr Metab (Lond). 18:64. PubMed
  147. Guo Y, et al. 2021. Nat Immunol. 22:746. PubMed
  148. Rivera CA, et al. 2022. Immunity. 55:129. PubMed
  149. Awida Z, et al. 2021. Int J Mol Sci. 23:. PubMed
  150. Zhang S, et al. 2016. PLoS Pathog. 12: 1005617. PubMed
  151. L M, et al. 2016. Brain. 139: 1939-1957. PubMed
  152. Tian T, et al. 2020. Cancer Immunol Res. 660:8. PubMed
  153. Hilt ZT, et al. 2019. JCI Insight. 4:5. PubMed
  154. Krotova K, et al. 2019. Mol Ther Oncolytics. 15:166. PubMed
  155. Lai NY, et al. 2020. Cell. 180:33:00. PubMed
  156. Kanaya T, et al. 2008. Am J Physiol Gastrointest Liver Physiol. 295:273. PubMed
  157. Amatruda M, et al. 2022. Brain Commun. 4:fcac044. PubMed
  158. Aggarwal N, et al. 2021. Cell Rep. 37:110170. PubMed
RRID
AB_312670 (BioLegend Cat. No. 100305)
AB_312671 (BioLegend Cat. No. 100306)

Antigen Details

Structure
Ig superfamily, forms CD3/TCR complex with CD3δ, γ and ζ subunits and TCR (α/β and γ/δ), 20 kD
Distribution

Thymocytes (differentiation dependent), mature T cells, NK-T cells

Function
TCR signal transduction, T cell activation, antigen recognition
Ligand/Receptor
Peptide antigen/MHC-complex
Cell Type
NKT cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules, TCRs
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Davis MM. 1990. Annu. Rev. Biochem. 59:475.
3. Weiss A, et al. 1994. Cell 76:263.

Gene ID
12501 View all products for this Gene ID
UniProt
View information about CD3epsilon on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11-30-2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account