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APC/Cyanine7 anti-human CD8 Antibody

Pricing & Availability
Clone
SK1 (See other available formats)
Regulatory Status
RUO
Other Names
T8, Leu2
Isotype
Mouse IgG1, κ
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Product Citations
publications
SK1_APCCyanine7_CD8_Antibody_032019_updated.png
Human peripheral blood lymphocytes were stained with CD8 (clone SK1) APC/Cyanine7 (filled histogram) or Mouse IgG1, κ APC/Cyanine7 isotype control (open histogram)
  • SK1_APCCyanine7_CD8_Antibody_032019_updated.png
    Human peripheral blood lymphocytes were stained with CD8 (clone SK1) APC/Cyanine7 (filled histogram) or Mouse IgG1, κ APC/Cyanine7 isotype control (open histogram)
See APC/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability Save
344713 25 tests 111€
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344714 100 tests 229€
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Description

CD8a is a 32-34 kD type I glycoprotein. It forms a homodimer (CD8a/a) or heterodimer (CD8a/b) with CD8b. CD8, also known as T8 and Leu2, is a member of the immunoglobulin superfamily found on the majority of thymocytes, a subset of peripheral blood T cells, and NK cells (which express almost exclusively CD8a homodimers). CD8 acts as a co-receptor with MHC class I-restricted T cell receptors in antigen recognition and T cell activation and has been shown to play a role in thymic differentiation. Two domains in CD8a are important for function: the extracellular IgSF domain binds the α3 domain of MHC class I and the cytoplasmic CXCP motif binds the tyrosine kinase p56 Lck.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human,Cynomolgus,Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

Clone SK1 recognizes the a chain of CD8. Additional reported applications (for the relevant formats) include: proteogenomics8, immunohistochemistry of acetone-fixed frozen tissue sections, and spatial biology (IBEX)9,10. This clone was tested in-house and does not demonstrate utility for formalin-fixed paraffin-embedded (FFPE) human tonsil sections.

Additional Product Notes
BioLegend is in the process of converting the name APC/Cy7 to APC/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our APC/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References
  1. Ledbetter JA, et al. 1981. J. Exp. Med. 153:310.
  2. Campanelli R, et al. 2002. Intl. Immunol. 14:39.
  3. Evans RL, et al. 1981. Immunol. 78:544.
  4. Wooldridge L, et al. 2005. J. Bio. Chem. 280:27491.
  5. Ch'el IL, et al. 2011. J Exp Med. 208:633. PubMed
  6. Carbone A, et al. 1999. Blood 93:2319. (IHC-F)
  7. Ahmed A, et al. 2001. J. Pathol. 193:383. (IHC)
  8. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
  9. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  10. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Chen J, et al. 2017. Sci Rep. 10.1038/s41598-017-11056-9. PubMed
  2. Eri Yamada et al. 2018. Cell host & microbe. 23(1):110-120 . PubMed
  3. Kubo M, et al. 2018. Oncol Rep. 39:417. PubMed
  4. Washburn ML, et al. 2019. J Immunol. 203:1897. PubMed
  5. Wang B, et al. 2018. Mol Ther Nucleic Acids. 0.548611111. PubMed
  6. Bochem J, et al. 2019. PLoS One. 14:e0221301. PubMed
  7. Hassounah NB, et al. 2019. Cancer Immunol Immunother. 68:407. PubMed
  8. de Macedo Abdo L, et al. 2020. Oncoimmunology. 9:1752592. PubMed
  9. Chiu YL, et al. 2018. Immun Ageing. 15:27. PubMed
  10. Zhu Y, et al. 2019. Cell Stem Cell. 25:542. PubMed
  11. Pucino V, et al. 2020. Cell Metabolism. 30(6):1055-1074.e8.. PubMed
  12. Bhattacharya S, et al. 2020. Front Bioeng Biotechnol. 1.055555556. PubMed
  13. Bego M, et al. 2012. J Virol. 86:3513. PubMed
  14. Argüello RJ, et al. 2012. PLoS One. 7:e35966. PubMed
  15. Weinberg A, et al. 2015. PLoS One. 10:122431. PubMed
  16. Lieberman L, et al. 2016. Neurology. 86: 375 - 381. PubMed
  17. Faust HJ, et al. 2020. J Clin Invest. 130:5493. PubMed
  18. Hood SP, et al. 2020. Elife. 9:00. PubMed
  19. Pierini S, et al. 2020. JCI Insight. 5:00. PubMed
  20. Kang L, et al. 2020. Exp Hematol Oncol. 9:11. PubMed
  21. Jiang Y, et al. 2020. Clin Immunol. 218:108516. PubMed
  22. Le J, et al. 2020. Immunity. 52(6):1105-1118.e9. PubMed
  23. Alter G, et al. 2020. Cell. 183(1):185-196.e14. PubMed
  24. Graham C, et al. 2021. Immunity. 54(6):1276-1289.e6. PubMed
  25. Goenka A, et al. 2021. Cell Reports Medicine. :100327. PubMed
  26. Loo Yau H, et al. 2021. STAR Protocols. 2(2):100549. PubMed
  27. Cheng Y, et al. 2021. Immunity. 54(8):1825-1840.e7. PubMed
  28. Ziegler CGK, et al. 2021. Cell. 184(18):4713-4733.e22. PubMed
  29. Wang J, et al. 2020. Cancer Immunol Res. 794:8. PubMed
  30. Such L, et al. 2020. J Clin Invest. 4266:130. PubMed
  31. Yang JY, et al. 2020. J Cell Mol Med. . PubMed
  32. Xun X, et al. 2021. Am J Transl Res. 13:4360. PubMed
  33. Ho CH, et al. 2021. Arthritis Res Ther. 23:199. PubMed
  34. Porsche CE, et al. 2021. JCI Insight. 6:. PubMed
  35. Li YR, et al. 2021. Cell Rep Med. 2:100449. PubMed
  36. Emmons TR, et al. 2021. Cancer Immunol Res. 9:790. PubMed
  37. Qing G, et al. 2021. Front Cell Dev Biol. 9:761300. PubMed
  38. Zebley CC, et al. 2021. Cell Rep. 37:110079. PubMed
  39. Li M, et al. 2022. Sci Rep. 12:378. PubMed
  40. Song C, et al. 2022. Stem Cell Res Ther. 13:48. PubMed
  41. Zebley CC, et al. 2021. Cell Rep. 37:109796. PubMed
  42. Biswas S, et al. 2022. Nat Commun. 13:2995. PubMed
  43. Elias G, et al. 2022. Elife. 11:. PubMed
  44. Schoutrop E, et al. 2022. Oncoimmunology. 11:2093426. PubMed
  45. Zhong M, et al. 2022. Cell Death Dis. 13:671. PubMed
  46. Diamantopoulos PT, et al. 2022. Cancers (Basel). 14:. PubMed
  47. Shi X, et al. 2022. NPJ Precis Oncol. 6:58. PubMed
  48. Smith KJ, et al. 2022. PLoS Biol. 20:e3001554. PubMed
  49. Muller YD, et al. 2021. Front Immunol. 12:639818. PubMed
  50. Grivas A, et al. 2022. Front Immunol. 13:964274. PubMed
  51. Zhou Y, et al. 2021. Methods Mol Biol. 2388:35. PubMed
  52. Balta E, et al. 2023. Front Immunol. 13:1063313. PubMed
  53. Larivière L, et al. 2023. Int J Pharm X. 5:100157. PubMed
  54. Zhong J, et al. 2023. Front Immunol. 13:1081889. PubMed
  55. Khojandi N, et al. 2021. Cancer Immunol Res. 9:214. PubMed
  56. Yen M, et al. 2022. Cell. 185:1414. PubMed
  57. Duhalde Vega M, et al. 2022. Sci Adv. 8:eabn6545. PubMed
  58. Zhao Q, et al. 2023. Signal Transduct Target Ther. 8:40. PubMed
  59. Tan X, et al. 2023. Adv Sci (Weinh). 10:e2206768. PubMed
  60. Segaliny AI, et al. 2023. Commun Biol. 6:380. PubMed
  61. Stitzlein LM, et al. 2023. Front Neurol. 14:1112207. PubMed
  62. Wu X, et al. 2023. iScience. 26:106559. PubMed
RRID
AB_2044005 (BioLegend Cat. No. 344713)
AB_2044006 (BioLegend Cat. No. 344714)

Antigen Details

Structure
Ig superfamily, homodimer or heterodimer with CD8b, 32-34 kD
Distribution

Majority of thymocytes, T cell subset, NK cells

Function
MHC class I co-receptor, thymic differentiation, T cell activation
Ligand/Receptor
MHC Class I molecules
Cell Type
NK cells, T cells, Thymocytes
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Barclay N, et al. 1993. The Leucocyte Antigen FactsBook. Academic Press Inc. San Diego.

Gene ID
925 View all products for this Gene ID
UniProt
View information about CD8 on UniProt.org
Go To Top Version: 8    Revision Date: 03-20-2019

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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