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PerCP anti-human CD3 Antibody

Pricing & Availability
Clone
UCHT1 (See other available formats)
Regulatory Status
RUO
Workshop
III 471
Other Names
T3, CD3ε
Isotype
Mouse IgG1, κ
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Product Citations
publications
UCHT1_PerCP_122607
Human peripheral blood lymphocytes stained with UCHT1 PerCP
  • UCHT1_PerCP_122607
    Human peripheral blood lymphocytes stained with UCHT1 PerCP
See PerCP spectral data
Cat # Size Price Quantity Check Availability Save
300427 25 tests 123€
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300428 100 tests 268€
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Description

CD3ε is a 20 kD chain of the CD3/T-cell receptor (TCR) complex which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T-cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T cells, NKT cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The CD3 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PerCP has a maximum absorption of 482 nm and a maximum emission of 675 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections4,6,7 and formalin-fixed paraffin-embedded sections11, immunoprecipitation1, activation of T cells2,3,5, Western blotting9, and spatial biology (IBEX)16,17. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 300413, 300414, and 300432). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 300437, 300438, 300465, 300466, 300473, 300474) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).

Application References
  1. Salmeron A, et al. 1991. J. Immunol. 147:3047. (IP)
  2. Graves J, et al. 1991. J. Immunol. 146:2102. (Activ)
  3. Lafont V, et al. 2000. J. Biol. Chem. 275:19282. (Activ)
  4. Ryschich E, et al. 2003. Tissue Antigens 62:48. (IHC)
  5. Thompson AG, et al. 2004. J. Immunol. 173:1671. (Activ)
  6. Sakkas LI, et al. 1998. Clin. Diagn. Lab. Immun. 5:430. (IHC)
  7. Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)
  8. Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
  9. Van Dongen JJM, et al. 1988. Blood 71:603. (WB)
  10. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  11. Pollard, K. et al. 1987. J. Histochem. Cytochem. 35:1329. (IHC)
  12. Luckashenak N, et al. 2013. J. Immunol. 190:27. PubMed
  13. Laurent AJ, et al. 2014. PLoS One. 9:103683. PubMed
  14. Li J, et al. 2015. Cancer Res. 75:508. PubMed
  15. Stoeckius M, et al. 2017. Nat. Methods. 14:865-868. (PG)
  16. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  17. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Hydes T, et al. 2017. Immun Inflamm Dis. 10.1002/iid3.190. PubMed
  2. Tognarelli S, et al. 2018. Front Immunol. 9:2743. PubMed
  3. Salvany‐Celades M et al. 2019. Cell Rep. 27(9):2537-2547 . PubMed
  4. Wang W, et al. 2019. Cell Rep. 28:486. PubMed
  5. Leng T, et al. 2019. Cell Rep. 28:3077. PubMed
  6. Ahmed R et al. 2019. Cell. 177(6):1583-1599 . PubMed
  7. Urlaub D, et al. 2017. J Immunol. 198:1944. PubMed
  8. Barra NG, et al. 2017. J Cancer Prev. 1.097222222. PubMed
  9. Cooper GE, et al. 2018. Front Immunol. 9:1671. PubMed
  10. Kim N, et al. 2020. Nat Commun. 2.045138889. PubMed
  11. Veerdonk F, et al. 2010. J Leukoc Biol. 88:227. PubMed
  12. Teirlinck A, et al. 2011. PLoS One. 7:e1002389. PubMed
  13. Alexander T, et al. 2013. Ann Rheum Dis. 72:1549. PubMed
  14. Livingston K, et al. 2013. J Immunol Methods. 390:18. PubMed
  15. Dannenmann S, et al. 2013. Oncoimmunology. 2:23562. PubMed
  16. Bartholomaeus P, et al. 2014. J Immunol. 192:2091. PubMed
  17. Obiero J, et al. 2015. Infect Immun . 83:2185. PubMed
  18. D, et al. 2016. Tuberculosis. 95: 470-475. PubMed
  19. Rother S, et al. 2015. PLoS One. 10: 0135682. PubMed
  20. Groen B, et al. 2015. Sci Rep. 5: 13618. PubMed
  21. Bigler M, et al. 2015. PLoS One. 10: 0145635. PubMed
  22. Bastiaens G, et al. 2016. Am J Trop Med Hyg. 94: 663 - 673. PubMed
  23. Meermeier E, et al. 2016. Nat Commun. 7:12506. PubMed
  24. Gerstner S, et al. 2016. J Leukoc Biol. 100(6):1297-1310. PubMed
  25. Linder A, et al. 2020. EMBO J. 39:e105071. PubMed
  26. Zhou J, et al. 2020. Oncol Lett. 1.336111111. PubMed
  27. Wenthe J, et al. 2021. Cancer Immunology Immunotherapy. . PubMed
  28. FitzPatrick MEB, et al. 2021. Cell Rep. 34:108661. PubMed
  29. Sananez I, et al. 2021. EBioMedicine. 72:103615. PubMed
  30. Wendisch D, et al. 2021. Cell. 184:6243. PubMed
  31. Dean JW, et al. 2020. J Autoimmun. 108:102417. PubMed
  32. Zuccato C, et al. 2022. Ther Adv Hematol. 13:20406207221100648. PubMed
  33. Fisher JG, et al. 2021. Front Oncol. 11:785635. PubMed
  34. Reitinger C, et al. 2022. Front Immunol. 13:970290. PubMed
  35. Lorvik KB, et al. 2021. Front Immunol. 12:744155. PubMed
  36. Jaiswal A, et al. 2022. Cancer Cell. 40:524. PubMed
  37. Rosenberg JM, et al. 2022. Med (N Y). 3:42. PubMed
  38. Terahara K, et al. 2022. iScience. 25:104959. PubMed
  39. Lee H, et al. 2023. Tuberc Respir Dis (Seoul). . PubMed
RRID
AB_893300 (BioLegend Cat. No. 300427)
AB_893298 (BioLegend Cat. No. 300428)

Antigen Details

Structure
Ig superfamily, with the subunits of CD3γ, CD3δ, CD3ζ (CD247) and TCR (α/β or γ/δ) forms CD3/TCR complex, 20 kD
Distribution

Mature T and NK T cells, thymocyte differentiation

Function
Antigen recognition, signal transduction, T cell activation
Ligand/Receptor
Peptide antigen bound to MHC
Cell Type
NKT cells, T cells, Thymocytes, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, TCRs
Antigen References

1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.
2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329.
3. Lanier L, et al. 1986. J. Immunol. 137:2501-2507.

Gene ID
916 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org

Related FAQs

How stable is PerCP/Cy5.5 tandem as compared to PerCP alone?

PerCP/Cy5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 1    Revision Date: 11-30-2012

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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