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PE/Cyanine7 anti-human CD16 Antibody

Pricing & Availability
Clone
3G8 (See other available formats)
Regulatory Status
RUO
Workshop
V NK80
Other Names
FcγRIII, Fc gamma receptor, Fc gamma receptor 3
Isotype
Mouse IgG1, κ
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Product Citations
publications
3G8
Human peripheral blood lymphocytes stained with 3G8 PE/Cyanine5
  • 3G8
    Human peripheral blood lymphocytes stained with 3G8 PE/Cyanine5
See PE/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability Save
302015 25 tests 115€
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302016 100 tests 234€
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Description

CD16 is known as low affinity IgG receptor III (FcγRIII). It is expressed as two distinct forms (CD16a and CD16b). CD16a (FcγRIIIA) is a 50-65 kD polypeptide-anchored transmembrane protein. It is expressed on the surface of NK cells, activated monocytes, macrophages, and placental trophoblasts in humans. CD16b (FcγRIIIB) is a 48 kD glycosylphosphatidylinositol (GPI)-anchored protein. Its extracellular domain is over 95% homologous to that of CD16a, and it is expressed specifically on neutrophils. CD16 binds aggregated IgG or IgG-antigen complex which functions in NK cell activation, phagocytosis, and antibody-dependent cell-mediated cytotoxicity (ADCC).

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human,Cynomolgus,Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human PMN cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The 3G8 antibody clone blocks neutrophil phagocytosis and stimulates NK cell proliferation. It has been reported that this clone interacts with the FcγRIIa and FcγRIIIb receptors causing neutrophil activation and aggregation18. Due to this phenomenon staining in whole blood may cause a reduction in the number of granulocytes or alter their scatter profile.

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections6, immunoprecipitation3, stimulation of NK cell proliferation4, blocking of phagocytosis5, and blocking of immunoglobulin binding to FcγRIII7,8. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 302049, 302050, 302057, 302058).

Additional Product Notes
BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References
  1. Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  2. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  3. Edberg J, et al. 1997. J. Immunol. 159:3849. (IP)
  4. Hoshino S, et al. 1991. Blood 78:3232. (Stim)
  5. Tamm A, et al. 1996. Immunol. 157:1576. (Block)
  6. Da Silva DM, et al. 2001. Int. Immunol. 13:633. (IHC)
  7. Holl V, et al. 2004. J. Immunol. 173:6274. (Block)
  8. Hober D, et al. 2002. J. Gen. Virol. 83:2169. (Block)
  9. Brainard DM, et al. 2009. J. Virol. 83:7305. PubMed
  10. Smed-Sörensen A, et al. 2008. Blood 111:5037. (Block) PubMed
  11. Timmerman KL, et al. 2008. J. Leukoc. Biol. 84:1271. (FC) PubMed
  12. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  13. Rout N, et al. 2010. PLoS One 5:e9787. (FC)
  14. Kim WK, et al. 2006. Am. J. Pathol. 168:822. (FC)
  15. Boltz A, et al. 2011. J. Biol Chem. 286:21896. PubMed
  16. Wu Z, et al. 2013. J. Virol. 87:7717. PubMed
  17. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
  18. Vossebeld PJ, et al. 1997. Biochem J. 323:87-94 (Stim)
Product Citations
  1. Lee J, et al. 2017. PLoS One.. 10.1371/journal.pone.0183594. PubMed
  2. Loelius SG et al. 2018. Current protocols in toxicology. 76(1):e46 . PubMed
  3. Walk J, et al. 2019. Nat Commun. 10:874. PubMed
  4. Kong XF, et al. 2018. Nat Immunol. 19:973. PubMed
  5. He W et al. 2018. Immunity. 49(6):1175-1190 . PubMed
  6. Dos Santos JC, et al. 2019. Cell Rep. 28:2659. PubMed
  7. Hunter S, et al. 2018. J Hepatol. 69:654. PubMed
  8. Escudero–Pérez B, et al. 2019. JCI Insight. 4:e126070. PubMed
  9. Jackson-Jones LH, et al. 2020. Immunity. 52:700. PubMed
  10. Serra EG, et al. 2020. Nat Commun. 11:995. PubMed
  11. Nafria M, et al. 2020. Cell Rep. 31:107691. PubMed
  12. Aguilar–Briseñ o JA, et al. 2020. Nat Commun. 2.664583333. PubMed
  13. Cirovic B, et al. 2020. Cell Host & Microbe. 28(2):322-334. PubMed
  14. Evans RDR, et al. 2020. Nat Commun. 3.491666667. PubMed
  15. Brainard D, et al. 2009. J Virol. 83:7305. PubMed
  16. Wilson E, et al. 2014. J Infect Dis. 210:1396. PubMed
  17. Mohanty S, et al. 2015. J Infect Dis. 211:1174. PubMed
  18. Collison J, et al. 2015. J Immunol. 195: 1162-1170. PubMed
  19. Llewellyn D, et al. 2015. Sci Rep. 5: 14081. PubMed
  20. Demers K, et al. 2016. PLoS Pathog. 12: 1005805. PubMed
  21. Laing AG, et al. 2020. Nat Med. 26:1623. PubMed
  22. Nafria M, et al. 2020. STAR Protoc. 1:100130. PubMed
  23. Riquelme SA, et al. 2020. Cell Metabolism. 31(6):1091-1106.e6. PubMed
  24. Abdul-Jawad S, et al. 2021. Cancer Cell. 39(2):257-275.e6. PubMed
  25. Golebski K, et al. 2021. Immunity. 54(2):291-307.e7. PubMed
  26. Li M, et al. 2020. Virol Sin. 35:588. PubMed
  27. Mol S, et al. 2021. Int J Mol Sci. 22:. PubMed
  28. Chen H, et al. 2021. Mol Ther Methods Clin Dev. 23:108. PubMed
  29. Cillo AR, et al. 2021. Cell Rep Med. 2:100476. PubMed
  30. Diamantopoulos PT, et al. 2022. Cancers (Basel). 14:. PubMed
  31. Pappa V, et al. 2021. J Neuroimmunol. 353:577524. PubMed
  32. Xu L, et al. 2022. Front Oncol. 12:967982. PubMed
  33. Castellano E, et al. 2022. Front Immunol. 13:970931. PubMed
  34. Sefik E, et al. 2022. Nature. 606:585. PubMed
  35. Hong Y, et al. 2022. J Virol Methods. 307:114564. PubMed
  36. Riemann D, et al. 2023. Biomolecules. 13:. PubMed
  37. Mauracher LM, et al. 2023. Res Pract Thromb Haemost. 7:100126. PubMed
RRID
AB_314215 (BioLegend Cat. No. 302015)
AB_314216 (BioLegend Cat. No. 302016)

Antigen Details

Structure
Ig superfamily, transmembrane form (50-65 kD) or GPI-linked form (48 kD)
Distribution

NK cells, activated monocytes, macrophages, neutrophils

Function
Low affinity IgG Fc receptor, phagocytosis, ADCC
Ligand/Receptor
Aggregated IgG, IgG-antigen complex
Cell Type
Dendritic cells, Macrophages, Monocytes, Neutrophils, NK cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, Fc Receptors
Antigen References

1. Fleit H, et al. 1982. P. Natl. Acad. Sci. USA 79:3275.
2. Stroncek D, et al. 1991. Blood 77:1572.
3. Wirthmueller U, et al. 1992. J. Exp. Med. 175:1381.

Gene ID
2214 View all products for this Gene ID
UniProt
View information about CD16 on UniProt.org

Related FAQs

Is our human Trustain FcX™ (cat# 422302) compatible with anti human CD16, CD32 and CD64 clones 3G8, FUN-2 and 10.1 respectively?

Yes

Go To Top Version: 2    Revision Date: 05-23-2014

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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