Loading...

Purified anti-human CD69 Antibody

Pricing & Availability
Clone
FN50 (See other available formats)
Regulatory Status
RUO
Workshop
IV A91
Other Names
Very Early Activation Antigen (VEA), Activation inducer molecule (AIM)
Isotype
Mouse IgG1, κ
Ave. Rating
Submit a Review
Product Citations
publications
FN50_Purified_070207
PHA-activated human peripheral blood lymphocytes stained with purified FN50, followed by anti-mouse IgGs FITC
  • FN50_Purified_070207
    PHA-activated human peripheral blood lymphocytes stained with purified FN50, followed by anti-mouse IgGs FITC
Cat # Size Price Quantity Check Availability Save
310902 100 µg 48€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD69 is a 27-33 kD type II transmembrane protein also known as activation inducer molecule (AIM), very early activation antigen (VEA), and MLR3. It is a member of the C-type lectin family, expressed as a disulfide-linked homodimer. Other members of this receptor family include NKG2, NKR-P1 CD94, and Ly49. CD69 is transiently expressed on activated leukocytes including T cells, thymocytes, B cells, NK cells, neutrophils, and eosinophils. CD69 is constitutively expressed by a subset of medullary mature thymocytes, platelets, mantle B cells, and certain CD4+ T cells in germinal centers of normal lymph nodes. CD69 is involved in early events of lymphocyte, monocyte, and platelet activation, and has a functional role in redirected lysis mediated by activated NK cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
CyTOF® - Verified
IHC-F - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections2, immunofluorescence microscopy3, and spatial biology (IBEX)8,9.

Application References
  1. Knapp WB, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  2. Sakkas LI, et al. 1998. Clin. and Diag. Lab. Immunol. 5:430. (IHC)
  3. Kim JR, et al. 2005. BMC Immunol. 6:3. (IF)
  4. Verjans GM, et al. 2007. P. Natl. Acad. Sci. USA 104:3496.
  5. Lu H, et al. 2009. Toxicol Sci. 112:363. (FC) PubMed
  6. Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
  7. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  8. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  9. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Martins M, et al. 2017. PLoS Pathog. 10.1371/journal.ppat.1006529. PubMed
  2. Jones N, et al. 2017. Front Immunol. . 10.3389/fimmu.2017.01516. PubMed
  3. Del Alcazar D, et al. 2019. Cell Rep. 28:3047. PubMed
  4. Kitai Y, et al. 2017. J Immunol. 198:1649. PubMed
  5. Singh AK, et al. 2017. Front Immunol. 0.513888889. PubMed
  6. Bruno TC, et al. 2017. Cancer Immunol Res. 0.831944444. PubMed
  7. Matos TR, et al. 2017. J Clin Invest. 127:4031. PubMed
  8. Guo T, et al. 2018. J Immunol. 200:500. PubMed
  9. Brinkmann CR, et al. 2018. mSphere. 3:e00616. PubMed
  10. Meckiff BJ, et al. 2019. J Immunol. 203:1276. PubMed
  11. Wang W, et al. 2018. Cancer Cell. 34:757. PubMed
  12. Martin JC, et al. 2020. Cell. 178(6):1493-1508.e20.. PubMed
  13. Ardain A, et al. 2019. Nature. 570:528. PubMed
  14. Patel NP, et al. 2018. J Infect Dis. 218:S88. PubMed
  15. Chng MHY, et al. 2020. Immunity. 51(6):1119-1135.e5.. PubMed
  16. Martin–Fernandez M, et al. 2020. Cell Reports. 31(6):107633. PubMed
  17. Gruber CN, et al. 2020. Cell. 183:982. PubMed
  18. Mishra A, et al. 2021. Cell. 184(13):3394-3409.e20. PubMed
  19. Taft J, et al. 2021. Cell. 184(17):4447-4463.e20. PubMed
  20. Yang Q, et al. 2020. J Immunol. 2331:204. PubMed
  21. Rouers A, et al. 2021. Cell Rep Med. 2:100278. PubMed
  22. Chiou SH, et al. 2021. Immunity. 54:586. PubMed
  23. Ehling RA, et al. 2022. Cell Rep. 38:110242. PubMed
  24. NULL, et al. 2022. Cell. 185:916. PubMed
  25. O'Boyle KC, et al. 2020. Methods Mol Biol. 2111:1. PubMed
  26. Sattler A, et al. 2021. Am J Transplant. 21:87. PubMed
  27. Lu X, et al. 2021. J Exp Med. 218: . PubMed
  28. McCarthy EE, et al. 2022. Cell Rep. 39:110815. PubMed
  29. Poon MML, et al. 2023. Nat Immunol. 24:309. PubMed
  30. Si W, et al. 2023. iScience. 26:106529. PubMed
RRID
AB_314837 (BioLegend Cat. No. 310902)

Antigen Details

Structure
C-type lectin, type II glycoprotein, 28/32 kD
Distribution

Activated T cells, B cells, NK cells, granulocytes, thymocytes, platelets, Langerhans cells

Function
Lymphocyte, monocyte, and platelet activation, NK cell killing
Cell Type
B cells, Granulocytes, Langerhans cells, NK cells, Platelets, T cells, Thymocytes, Tregs
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
2. Testi R, et al. 1994. Immunol. Today 15:479.

Gene ID
969 View all products for this Gene ID
UniProt
View information about CD69 on UniProt.org
Go To Top Version: 2    Revision Date: 08-25-2022

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account