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Brilliant Violet 421™ anti-mouse CD133 Antibody

Pricing & Availability
Clone
315-2C11 (See other available formats)
Regulatory Status
RUO
Other Names
Prominin-1, AC133
Isotype
Rat IgG2a, λ
Ave. Rating
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Product Citations
publications
315-2C11_B421_CD133_Antibody_FC_1_080715
Mouse CD133 transfected cells were stained with CD133 (clone 315-2C11) Brilliant Violet 421™ (filled histogram) or rat IgG2a, κ Brilliant Violet 421™ isotype control (open histogram).
  • 315-2C11_B421_CD133_Antibody_FC_1_080715
    Mouse CD133 transfected cells were stained with CD133 (clone 315-2C11) Brilliant Violet 421™ (filled histogram) or rat IgG2a, κ Brilliant Violet 421™ isotype control (open histogram).
  • 315-2C11_B421_CD133_Antibody_FC_2_080715
    C57BL/6 mouse bone marrow cells were stained with CD117 PE and CD133 (clone 315-2C11) Brilliant Violet 421™ (top) or rat IgG2a, κ Brilliant Violet 421™ (bottom) isotype control.
  • 315-2C11_B421_CD133_Antibody_FC_3_080715
Compare all formats See Brilliant Violet 421™ spectral data
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141213 50 µg $292
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Description

CD133 (also known as prominin-1 and AC133) is a 120 kD pentaspan glycoprotein with 5 transmembrane domains which localize to cellular protrusions. CD133 was initially described as a surface antigen specific for human hematopoietic stem cells and as a marker for murine neuroepithelial cells and some embryonic epithelia. Later on, CD133 was found on other stem cells, including endothelial progenitor cells, glioblastomas, neuronal, and glial stem cells. In addition to hematopoietic stem cells, CD133 was found on cancer cells, such as some leukemia cells and brain tumor cells. It also has been detected at low levels in the kidney, pancreas, placenta, and fetal liver tissue. Although the biological function of CD133 is not completely understood, CD133 has been extensively used as a stem cell marker for normal and cancerous tissues.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
CD133-expressing-GFP-RBL2H3 plus CFA as adjuvant.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Zhang Y, et al. 2012. Diabetes. 61:2114. PubMed
RRID
AB_2566011 (BioLegend Cat. No. 141213)

Antigen Details

Structure
120 kD pentaspan transmembrane glycoprotein
Distribution
Hematopoietic stem cells, epithelial progenitor cells, leukemia cells, brain tumor cells, kidney, pancreas, placenta, and fetal liver cells
Function
Biological function unknown
Cell Type
Hematopoietic stem and progenitors, Epithelial cells, Leukemia, Neural Stem Cells
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Singh SK, et al. 2004. Nature 18:396.
2. Falini B, et al. 2005. New Engl. J. Med. 352:254.
3. Bonanno G, et al. 2004. Transfusion 44:1087.
4. Gaipa G, et al. 2002. Haematologica 87:347.
5. Wuchter C, et al. 2001. Haematologica 86:154.

Gene ID
19126 View all products for this Gene ID
UniProt
View information about CD133 on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 1    Revision Date: 08/17/2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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